Dr Dip. eng. Anna Nowicka

Doctors
Department of Cell Biology
(+48) 12 4253301 (wew./ext. 39)
a.nowicka@ifr-pan.edu.pl
Epigenetic factors that influence on the process of microspore embryogenesis • DNA damage repair mechanisms in plants

Publications

more publications

Projects

 EDUCATION AND ACADEMIC DEGREES

2013 doctor’s degree in agricultural sciences; speciality: genetics and plant breeding, at UAK
2009-2010 postgraduate study at The Pedagogy and Psychology Centre, Krakow Uniwersity of Technology
2007-2011 PhD student in Department of Genetics, Plant Breeding and Seed Science, University  of Agriculture in Krakow (UAK)
2007 master and engineer's degree in applied biotechnology, IDB AUK
2002-2007 study at The Interfaculty Department of Biotechnology, Agricultural University (now University of Agriculture) in Krakow (IDB AUK)


PROFESSIONAL EXPERIENCE

2015-2016 DAAD post-doc at the Max Planck Institute for Plant Breeding Research (Cologne, Germany)
since 2013 post-doc at the Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences in Krakow (IPP PAS)


RESEARCH AREAS

Epigenetic factors that influence on the process of microspore embryogenesis.
Organization of plant nuclear genome at the chromosomal level. 
DNA damage repair mechanisms in plants.


PRACTICAL SKILLS

Cytogenetics/molecular cytogenetics

Mitotic and meiotic chromosome preparation. Nuclei preparation.
Probe labeling.
Fluorescence in situ hybridization (FISH). 
Genomic in situ hybridization (GISH).
5-methylcytosine (5mC) and histone immunodetection.
Image capturing and processing. 
Chromosome measurements. Construction of the karyotypes and idiograms.
Flow cytometry (FAC).

Molecular biology/bioinformatics

Plasmid preparation, genomic DNA extraction, RNA extraction, ligation, transformation, PCR, RT-PCR, RT-qPCR, genotyping, electrophoresis techniques.

New generation sequencing (NGS) including RNA-seq, bio-softwares and biological databases. 

In vitro cultures

Methods of in vitro cultures.
Transformation mediated by Agrobacterium tumefaciens and Agrobacterium rhizogenes.


PUBLICATIONS till 2014

Macko-Podgorni A., Nowicka A., Grzebelus E., Simon P.W., Grzebelus D. 2013. DcSto: carrot Stowaway-like elements are abundant, diverse, and polymorphic. Genetica, 141:255-267. doi 10.1007/s10709-013-9725-6.


Nowicka A., Grzebelus E., Grzebelus D. 2012. Fluorescent in situ hybridization with arbitrarily amplified DNA fragments differentiates carrot (Daucus carota L.) chromosomes. Genome, 55: 1-9. Read more.


Czernicka M., Nowicka A., Klein M., Muras P., Grzebelus E. 2011. A cytogenetic approach for the evaluation of hybryd state in Rhododendrons. American Rhododendron Society, 6(1): 10-13. 


Czernicka M., Nowicka A., Klein M., Muras P., Grzebelus E. 2010. Paternity determination of interspecific rhododendron hybrids by genomic in situ hybridization (GISH). Genome, 53: 277-284. Read more.

 

Publications with citations and indexes available on the following websites: