Dr hab Ewa Dubas

Associate Professors
Department of Cell Biology
(+48) 12 4253301 (wew./ext. 39)
e.dubas@ifr-pan.edu.pl
The mechanisms of gametogenesis, androgenesis and embryo formation (cytoskeleton, endogenous hormonal-control, embryo polarity) in crops • double haploids • pathogenesis

Projects

DIPLOMAS, DEGREES

2015-

2007-

2001-

Associate Professor in Agriculture, Agriculture University, Kraków, Poland

PhD in Biology, Jagiellonian University, Kraków, Poland

M.Sc in Biology – Department of Plant Cytology and Embryology, Jagiellonian University, Krakow, Poland

 

 

 

 

EMPLOYMENT IN ACADEMIC INSTITUTIONS

2007-at present Adiunct [Assistant Professor], Department of Cell Biology in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow
2001-2007 PhD student, Department of Cell Biology in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow
2001-2007 Interdisciplinary Ph.D. Study in the Faculty of Biology and Earth Sciences, Jagiellonian University

 

 

 

MEMBERSHIP AND FUNCTIONS

2014 -2017

Polish deputy in Management Committee of COST FA1306 ‘The quest for tolerant varieties: Phenotyping at plant and cellular level’

2014

External expert evaluator of the international research project Fund for Scientific Research – FNRS

2011-2013  

Polish deputy in Management Committee of COST FA (Food and Agriculture) 0903 ‘Harnessing Plant Reproduction for Crop Improvement’

 

 

 

 

 

 

 

RESEARCH AREAS

  • Cell biology (cytoskeleton, cell cycle, auxin and abscisic acid  signaling, plasma membrane fluidity) in responses to abiotic and biotic stress.

  • Effect of stress on plant’s responses in gene and protein level, transgenesis, plant-growth regulators, in vitro tissue culture of cereals and Brassicaceae.

  • Pathogenesis - cytological and physiological analyses of the infection processes.

 

PRACTICAL SKILLS

  • In vitro culture: oil-seed rape microspore culture, cereal anther and microspore culture, immature embryo culture.

  • Tracing of molecules in plant material: analysis of alfa-tubulin, auxin and auxin efflux carrier proteins localization by immunocytochemistry, fluorescent and laser confocal microscopy (CLSM).

  • Tracing of the fungal infection process in plant material by cytology and microscopy.

  • Protein analyses: immunolocalisation of proteins in intact cells ('whole mount') and in sectioned material, protein isolation electrophoresis and western blotting.

  • Gene expression: RNA, DNA and pDNA isolation, PCR, qPCR, primer design.

  • Restriction analysis.

  • Bioinformatics: Sequence analysis, gene and protein sequence alignment: BLAST.

  • Chromosomes analyses: karyotype analyses, C-banding of chromosomes.

  • Quantitative and qualitative analysis of ROS and antioxidative enzyme activity: H2O2 measurement, SOD superoxide dismutase activity fluorometric measurements.

  • Quantitative analysis of antifungal enzyme activity: fluorometric assay to determine glucanase activity.

  • Microspores/Androgenic embryos transformation with Agrobacterium tumefaciens.

 

RESEARCH EXPERIENCE

Post doc projects. My Post Doc projects concentrated mainly on: (1) endogenous auxins as a triggers in double haploids (DHs) production in oilseed rape, (2) the role of polar auxin transport and auxin signalling in embryo development. Plant embryonic development involves differential distribution (gradients) of the plant signaling molecule auxin. These gradients are established and maintained by a directional, intercellular auxin transport called polar auxin transport. Polar auxin transport provides positional and directional information for many aspects of embryo development. B. napus microspore suspension is a suitable model system for such studies. By using combination of genetic, molecular, physiological and cell biology approaches I would like to address the key question: how plant hormones perform their respective roles in embryo development, with a special focus on hormonal interactions.

PhD project. My PhD project concentrated on early embryo development in microspore suspension in vitro cultures of Brassica napus. High yields of microspore-derived embryos MDE in suspension were reproducibly obtained according to the method elaborated previously by research team of dr Jan Custers PRI Wageningen, The Netherlands. I adopted the method and further optimised in my laboratory. MDE develop and differentiate in a way resembling zygotic embryos in almost every studied aspect, therefore I used them to study early stages of plant embryogenesis. The main goal of these studies was to study the development of androgenic embryos from the earliest stages (a single cell) onwards including suspensor formation. Studying those structures during zygotic development is very difficult due to ovular tissues that surround embryos. I focused on establishment of embryo polarity and the role of cytoskeleton during MDE differentiation tracing also mitochondria and amyloplasts positions, vacuolisation pattern, cell-wall formation and differentiation of embryo organs. My studies required optimalization of many methods and development of protocols of immunodetection and tracing of various structures in whole-mount (not sectioned) material.

               

 

PUBLICATIONS

                 

 Read more

 Selected papers listed by year with links to the publishers' websites: 

  1. Dubas E., Moravčíková J., Libantová J., Matušíková I., Benkova E., Żur I., Krzewska M. 2014. The influence of heat stress on auxin distribution in transgenic B. napus microspores and microspore derived embryos. Protoplasma 251:1077-1087. IF2013 3.171  Read more
  2. Dubas E., Custers J., Kieft H., Wędzony M., van Lammeren A.A.M. 2014. Characterization of polarity development through 2- and 3-D imaging during the initial phase of microspore embryogenesis in Brassica napus L. Protoplasma 251: 103-113. IF2013 3.171  Read more

  3. Wędzony M., Szechyńska-Hebda M., Żur I., Dubas E., Krzewska M. 2014. Tissue culture and regeneration: a prerequisite for alien gene transfer. In: Alien Gene Transfer in Crop Plants, Vol. 1. Innovations, Methods and Risk Assessment. Pratap A., Kumar, J. (Eds.), Springer, ISBN 978-1-4614-8584-1 Read more

  4. Żur I., Dubas E., Krzewska M., Janowiak F., Hura K., Pociecha E., Bączek-Kwinta R., Płażek A. 2014. Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis. Plant Cell, Tissue and Organ Culture (PCTOC). DOI: 10.1007/s11240-014-0515. IF2013: 2.61  Read more

  5. Żur I.,.Dubas E*., Krzewska M., Sánchez-Díaz R.A., Castillo A.M., Vallés M.P. 2014. Changes in gene expression patterns associated with microspore embryogenesis in hexaploid triticale (× Triticosecale Wittm.)", submitted to Plant Cell, Tissue and Organ Culture (PCTOC) 116(2):261-267. IF2013 2.612  Read more

  6. Żur I.,.Dubas E., Krzewska M., Waligórski P., Dziurka M., Janowiak F. 2014Hormonal requirements for effective induction of microspore embryogenesis in triticale (×Triticosecale Wittm.) anther cultures. Plant Cell Rep DOI 10.1007/s00299-014-1686-4MNiSW 35 pkt., IF2013 2,936  Strona wydawcy

  7. Dubas E., Janowiak F., Krzewska M., Hura T., Żur I. 2013. Endogenous ABA concentration and cytoplasmic membrane fluidity in microspores of oilseed rape (Brassica napus L.) genotypes differing in responsiveness to androgenesis induction. Plant Cell Rep 32:1465–1475. IF2013 2.936  Read more

  8. Pociecha E., Janowiak F., Dubas E., Żur I., Tokarz K., Kolasińska I., Płażek A. 2013. Progress of snow mould infection in crowns of winter rye (Secale cereale L.) is related to photosynthetic activity during cold acclimation. Plant Physiology and Biochemistry 70:360-367. IF2013: 2.352 Read more

  9. Żur I., Gołębiowska G., Dubas E., Golemiec E., Matušíková I., Libantová J., Moravčíková J. 2013. β-1,3-glucanase and chitinase activities in winter triticales during cold hardening and subsequent infection by Microdochium nivale. Biologia 68/2: 241-248. IF2013: 0.696 Read more

  10. Żur I., Dubas E., Słomka A., Dubert F., Kuta E., Płażek A. 2013. Failure of androgenesis in Miscanthus×giganteus in vitro culture of cytologically unbalanced microspores. Plant Reproduction 26(3):297-307. IF2012: 2.07   Read more

  1. Dubas E., Wędzony M., Custers J., Kieft H., van Lammeren A.A.M. 2012. Gametophytic development of Brassica napus pollen in vitro enables examination of cytoskeleton and nuclear movements. Protoplasma 249(2): 369-377. IF2013: 3.171  Read more
  2. Krzewska M., Czyczyło-Mysza I., Dubas E., Gołębiowska-Pikania G., Golemiec E., Stojałowski S., Chrupek M., Żur I. 2012. Quantitative trait loci associated with androgenic responsiveness in triticale (×Triticosecale Wittm.) anther culture. Plant Cell Reports 31:2099–2108. Plant Cell Reports 31:2099–2108. IF2013: 2.936  Read more

  3. Słomka A., Kuta E., Płażek A., Dubert F., Żur I., Dubas E., Kopeć P., Żurek G. 2012. Sterility of Miscanthus×giganteus results from hybrid incompatibility. Acta Biologica Cracoviensia Series Botanica 54/1: 1–8. IF2013: 0.662 Read more

  4. Żur I., Krzewska M., Dubas E., Gołębiowska-Pikania G., Janowiak F., Stojałowski S. 2012. Molecular mapping of loci associated with abscisic acid accumulation in triticale (×Triticosecale Wittm.) anthers in response to low temperature stress inducing androgenic development. Plant Growth Regulation 68(3): 483-492. IF2013: 1.625  Read more

  5. Dubas E., Custers J.B.M., Kieft H., Wędzony M., van Lammeren  A.A.M. 2011. Microtubule configurations and nuclear DNA synthesis in microspore derived embryos of Brassica napus cv Topas that mimic zygotic embryo development. Plant Cell Reports 30: 2105–2116. IF2013 : 2.936   Read more

  6. Dubas E., Golebiowska G., Zur I., Wedzony M. 2011. Microdochium nivale (Fr., Samuels & Hallett): cytological analysis of the infection process in triticale (x Triticosecale Wittm.). Acta Physiologiae Plantarum 33(2): 529-537. IF2013: 1.524  Read more

  7. Żur I.A., Dubas E., Pociecha E., Dubert F., Kolasińska I., Płażek A. 2011. Cytological analysis of infection process and the first defence responses induced in winter rye (Secale cereale L.) seedlings inoculated with Microdochium nivale. Physiological and Molecular Plant Pathology 76: 189-196. IF2013: 1.987 Read more

  8. Szechyńska-Hebda M., Wędzony M., Tyrka M., Gołębiowska G., Chrupek M., Czyczyło-Mysza I., Dubas E., Żur I., Golemiec E. 2011. Identifying QTL for cold-induced resistance to Microdochium nivale in winter triticale. Plant Genetic Resources: Characterization and utilization 9(2):296-299. IF2013: 1.057 Read more

  1. Dubas E., Wędzony M., Petrovska B., Salaj J., Żur I. 2010. Cell structural reorganization during induction of androgenesis in isolated microspore cultures of triticale (x Triticosecale Wittm.). Acta Biologica Cracoviensia Series Botanica 52/1: 73–86. IF2013: 0.662 Read more
  2. Dubas E., Marzec K., Płażek A. 2010. Cytological studies on Microdochium nivale infection in Lolium perenne (L.) plants. Zeszyty Problemowe Postępów Nauk Rolniczych PAN Zeszyt 545:389-401.

  3. Żur I., Dubas E., Golemiec E., Szechyńska-Hebda M., Gołębiowska G., Wędzony M. 2009. Stress-related variation in antioxidative enzymes activity and cell metabolism efficiency associated with embryogenesis induction in isolated microspore culture of triticale (xTriticosecale Wittm.). Plant Cell Reports 28:1279–1287. IF2013: 2.36  Read more

  4. Wędzony M., Forster B.P., Żur I., Golemiec E., Szechyńska-Hebda M., Dubas E., Gołębiowska G. 2009.  Progress in Doubled Haploid Technology in Higher Plants. In: Touraev A., Jain M., Forster B. (eds.): “Advances in Haploid Production in Higher Plants” © Springer Science + Business Media B.V., pp.1-35. IC 30 Read more

  1. Supena EDJ., Winarto B., Riksen T., Dubas E., van Lammeren A., Offringa R., Boutilier K., Custers J. 2008. Regeneration of zygotic-like microspore-derived embryos suggests an important role for the suspensor in early embryo patterning. Journal of Experimental Botany 59(4): 803-814. doi:10.1093/jxb/erm358.IF2013: 5.794  Read more

  2. Żur I., Dubas E., Golemiec E., Szechyńska-Hebda M., Janowiak F., Wędzony M. 2008. Stress-induced changes important for effective androgenic induction in isolated microspore culture of triticale (xTriticosecale Wittm.). Plant Cell, Tissue and Organ Culture 94:319-328.  Read more

  3. Szechyńska-Hebda M., Wędzony M., Dubas E., Kieft H., van Lammeren A. 2006. Visualisation of microtubules and actin filaments in fixed BY-2 suspension cells using an optimized whole mount immunolabeling protocol. Plant Cell Reports. 25 (8): 758 – 766. IF2013: 2.936  Read more

  4. Żur I., Godzisz I., Dubas E., Golemiec E., Wędzony M. 2006. The influence of cold pretreatment on androgenesis efficiency in isolated microspore culture of triticale (X Triticosecale Wittm.). Zeszyty Problemowe Postępów Nauk Rolniczych 509: 49-59.

 

PARTICIPATION IN RESEARCH PROJECTS 

NATIONAL  (RUNNING PROJECTS)

2014 - 2018

Identification of factors determine drought and frost resistance in winter barley (Hordeum vulgare L.)’. Biological progress, Task No. 26 (Appendix No. 9 to the Regulation of the Minister of Agriculture and Rural Development on the rate subsidy for various entities performing tasks for agriculture in May 18th, 2010 (Journal of Laws No. 91, Item 595, as amended); a role: researcher

2013 - 2014

Identification of drought induced signalling pathway in barley leaves’. Research national project No. G-1843 ; a role: researcher

2011 - 2014 

‘The role of endogenous auxins: Indole-3-acetic acid (IAA) and Indole-3-butyric acid (IBA) in auxin homeostasis and androgenic effectivness enhancement in oilseed rape (Brassica napus)’. Individual research national project No. 2011/01/D/NZ9/02547; a role: project coordinator

INTERNATIONAL (LAST 5 YEARS)

2010 - 2013

Development methods to obtain differentiated morphologically and physiologically giant Miscanthus plants (Miscanthus x giganteus) by gyno-and  androgenesis’. Special project 1780 COST in the framework in Action COST FA0903; a role: researcher

2009 - 2011

‘Localization of QTL for androgenic response on triticale (× Triticosecale Wittm.)’. Research project 548/N-COST/2009/0, COST Action FA0604: Triticeae genomics for the advancement of essential European crops (TritiGen); a role: researcher

2009 - 2011 

‘Physiological and biochemical indicators of winter rye resistance to Microdochium nivale’. Research project 592/N-COST/2009/0, COST Action FA0604: Triticeae genomics for the advancement of essential European crops (TritiGen); a role: researcher

BILATERAL

2011 -2013

‘N-butanol as a trigger inducing microspore embryogenesis in in vitro cultures of wheat (Triticum aestivum L.) and triticale (×Triticosecale Wittm.)’. Polish – Spanish research project (IPP PAS and Department of Genetics and Plant Breeding, EEAD-CSIC in Zaragoza); a role: researcher, coordinator one of task

2010- 2012

‘Auxin as signalling molecule in doubled haploid production of rape (Brassica napus var. oleifera)’. Polish-Belgian research project (IPP PAS and VIB in Ghent University); a role: coordinator

2010 - 2012 

‘Molecular markers in auxin gradient analysis in androgenic embryos of rape (Brassica napus var. oleifera)’. Polish – Slovac research project (IPP PAS and Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences); a role: coordinator

 

 

COOPERATION WITH FOREIGN SCIENTIFIC INSTITUTIONS

 
2012
  • Research fellowships at Plant Systems Biology Department (VIB), Ghent University, Ghent, Belgium; 4 weeks
  • Microspore embryogenesis in triticale (xTriticosecale Wittm.): assignment of genes to embryo formation" (individual research project, 2 weeks ) STMS of COST FA0903 “Harnessing plant reproduction for crop improvement” at EEAD-CSIC, Zaragoza, Spain, a role: the main researcher.
2011
  • Research fellowships at Plant Systems Biology Department (VIB), Ghent University, Ghent, Belgium; 3 weeks
  • Research  fellowship at The Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia; 1 week
  • Research fellowships at Plant Vegetal in (CSIC), Zaragoza in Spain; 2 weeks 
2010
  • Research fellowships at Plant Systems Biology Department (VIB), Ghent University, Ghent, Belgium; 1 week
  • Research  fellowship at The Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia; 1 week
  • Research ‘NOBEL’ fellowships 'Transformation of microspores and androgenic embryos of rapeseed (Brassica napus)” in frame of “Investment into science – Investment into future. Joint project of three scientific centers from Krakow, Olsztyn and Warsaw” at The Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia; 1 week
  • Research ‘SAIA’ fellowships 'Techniques of recombinant DNA’ (individual research project) at The Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia; 4 weeks.
  • Research fellowships at Plant Systems Biology Department (VIB), Ghent University, Ghent, Belgium.
2009
  • Research fellowships at The Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia.
2008
  • Research fellowships at The Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia.
2003-2004
  • Research fellowships at Wageningen University and Research Centre in the frame of FP5 project CROPSTRESS and COST 851 Action STSM mission.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

TEACHING EXPERIENCE

Pedagogical University of Krakow (Krakow) Contracted teaching hours (lectures and tutorials). Courses in the framework of the Postgraduate Studies with Molecular Biology and Biotechnology Elements at the Faculty of Geography and Biology of Pedagogical University in Krakow. Postgraduate studies were carried out within Task 14 ‘Activation of Studies with Molecular Biology and Biotechnology Elements’ in the project ‘Developing the Didactic Potential in the Pedagogical University of Krakow’ co-founded by the European Social Funds in the academic years 2009/10 i 2010/11.

Polish
Academy
of Sciences
in Krakow 
Lectures in the framework of the International Doctoral Studies of the Natural Sciences of the PAS in Krakow in 2014.

 

 

 

 

 

 

 

SUPERVISION OF M.Sc STUDENTS

- Place: The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences, Department of Cell Biology:

2007-2008

2010-2012

Supervision of the execution experiments for two M.Sc students in the Institute of Biology at the Pedagogical University in Krakow.

Supervision of the execution experiments for two M.Sc students in the Institute of Biology at the Pedagogical University in Krakow.

2013 MSc Supervisor of two students: M.Sc Anna Malinowska and M.Sc Monika Okarmus in the Pedagogical University in Krakow, Faculty of Geography and  Biology, Institute of Biology.

 

 

 

 

 

AWARDS AND PRIZES

2007- Doctoral dissertation with distinction at the Jagiellonian University in Krakow
2011-2013

Award from the Director of the Institute of Plant Physiology Polish Academy of Sciences for the highest number of publications in journals from the Philadelphia list in the years: 2011, 2012 and 2013 in the group of younger scientists.