Dr hab Piotr Waligórski

Role and mechanisms of mycorrhiza in plants and its contribution to alleviation of drought stress Proteomics of galls

Publications

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Projects

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EDUCATION AND CAREER

2018 - present - assistant at The Franciszek Gorski Institute of Plant Physiology, Polish Academy of Science, Krakow, Poland
2008 – 2018 - post-doc (adiunkt) at The Franciszek Gorski Institute of Plant Physiology, Polish Academy of Science, Krakow, Poland
2014 - present - head of The Joint Laboratory of Biotechnology and Enzyme Catalysis
2005 – 2014 - staff member at The Joint Laboratory of Biotechnology and Enzyme Catalysis
2010 - 2011 - Postdoctoral Researcher at Stanley S. Scott Cancer Center, Louisiana State University, New Orleans, USA
2004 – 2008 - assistant at The Franciszek Gorski Institute of Plant Physiology, Polish Academy of Science, Krakow, Poland
1998 – 2003 - Ph.D. Studies at the Faculty of Agriculture and Economics, Agricultural University, Krakow, Poland
1993 – 1998 - M.Sc. Studies at the Faculty of Biology, Jagiellonian University, Krakow, Poland, speciality: molecular biology


RESEARCH INTERESTS

Hormonal regulation of plant flowering.
Proteomics of galls.
Physiological role of silicon compounds.
Self-incompatibility of cabbage.
Arbuscular mycorrhiza and its link with hormonal regulation of plant physiology.
The application of modern techniques in plant physiology studies: high performance liquid chromatography, mass spectroscopy, capillary electrophoresis.

PRACTICAL SKILLS

Determination of the plant compounds (MS HPLC):
- auxines (IAA, IBA)
- cytokinines (kinetin, zeatin, their rybosides and others)
- abscisic acid (ABA)
- polyamines (putrescine, spermine, spermidine and others)
- sugars (glucose, fructose, sucrose, maltose and others)
- phenolic acids (salicylic acid, caffeic, cynnamic and others)
- carotenoids (violaxanthin, zeaxanthin, lutein and others)
- flavonoids
- tocochromanoles (tocopheroles and tocotrienoles)

Capillary Electrophoresis:
- proteins
- organic and inorganic anions

Proteomics
- 2D gel electrophoresis
- MALDI TOF protein identification
- ShotGun analyses

ELISA assay

Image analysis:
- good experience in LUCIA system

PARTICIPATION IN GRANTS
N N310 452638 - Możliwość zwiększenia efektywności androgenezy u pszenżyta (x Triticosecale Wittm.) poprzez kontrolę równowagi hormonalnej i natężenia stresu oksydacyjnego w pylnikach. Project leader: dr hab. Iwona Żur 2010 - 2013
621/N-COST/09/2010/0 - Aktywność poliamin i związków fenolowych w reakcji odporności na suszę glebową roślin motylkowych uprawianych na białko. Project leader: dr inż. Edyta Skrzypek, 2010 - 2012
2 P04G 109 28 – The influence of mycorrhizal fungi on biomass production and level of active compounds in Arnica montana L. Project leader: dr Anna Jurkiewicz, 23.03.2005-22.03.2008
CROPSTRESS QLAM-2001-00424 – Centre of Research on the Biology of Plants Subjected to Environmental Stress in Sustainable Agricultural Production. Project leader: dr hab. Maria Wędzony, 01.04.2003-30.03.2006
R12 045 02 – Development of methods of vegetable treating for increasing its global resistance, content of natural antioxidants and stabilization of crop quality during storage. Project leader: prof. dr hab. Zbigniew Miszalski
PL 023168 SAFIR – Safe and High Quality Food Production using Low Quality Waters and Improved Irrigation Systems and Management. 2006-2008
5 P06A 032 18 – The influence of coincidence between photosynthetic active radiation and temperature to gaining frost resistance in rape (Brassica napus var. oleifera) by growth rate changes during prehardening and dehardening. Project leader: dr eng. Marcin Rapacz, 01.01.2000-31.12.2002