Dr hab Franciszek Janowiak

Associate Professors
Department of Ecophysiology
(+48) 12 4253301 (wew./ext. 42)
f.janowiak@ifr-pan.edu.pl
Abscisic acid (ABA) • chilling stress • plant stress physiology • root-to-shoot communication • water use efficiency

Plant biologist specializing in stress physiology of crops, particularly in adaptation and defense mechanisms under adverse environmental conditions, including low temperature, drought, and flooding.


EDUCATION AND ACADEMIC DEGREES

1977-1982 study at the Faculty of Agronomy, Agricultural University (now University of Agriculture) in Kraków (FA AUK); semestral training in Switzerland

1982 master and engineer's degree in agricultural sciences, plant physiology at FA AUK

1992 doctor's degree in agricultural sciences, agronomics-plant physiology at FA AUK

2005 habilitation in the field of agricultural sciences, agronomics-plant physiology at FA AUK


PROFESSIONAL EXPERIENCE

1982-1992 research assistant and senior research assistant at the Department (now Institute) of Plant Physiology, Polish Academy of Sciences in Kraków (IPP PAS)

1988-1990 holder of DAAD fellowship at the Institute of General Botany (now Biocenter Klein Flottbek), University of Hamburg, Germany

1992-2006 adiunkt at IPP PAS

2006-2010 docent at IPP PAS

since 2010 associate professor of IPP PAS


MEMBERSHIP AND FUNCTIONS

Member of Scientific Council of IPP PAS (since 2006)

Head of Department of Ecophysiology in IPP PAS (since 2011)

Deputy Director for Scientific Research of IPP PAS (since June 2012)


RESEARCH AREAS

Stress physiology of plants – root to shoot communication, hormonal balance (abscisic acid (ABA), cytokinins, ethylene, and 1-aminocyclopropane-1-carboxylic acid (ACC, ethylene's precursor), water relations, photosynthetic efficiency and stomata status under low temperature (chilling, frost), drought and flooding stress conditions in various crop plants, especially maize, tomato, oil rape, and sorghum

Water use efficiency in plants – physiological grounds for improved water use efficiency by crop plants under common and alternating deficit watering

Abscisic acid (ABA) – role of ABA in plant reactions to abiotic and biotic stresses as well as in regulation of ability of cells and tissues to regenerate plants in vitro

Keywords of the research areas: abiotic stress • abscisic acid (ABA) • biotic stress • chilling • chlorophyll a fluorescence • deficit irrigation • DPPH radical scavenging method • drought • ELISA for ABA • flooding • frost • hormonal balance • low temperature • low-molecular antioxidants • maize (Zea mays L.) • oil rape (Brassica napus var. oleifera) • photosynthetic efficiency • plant hormones • root to shoot communication • sorghum (Sorghum bicolor (L.) Moench) • stomata status • stress physiology • tomato (Solanum lycopersicum L.) • total antioxidant activity • water relations • water use efficiency


METHODS USED

Enzyme immunomethods – measurement of abscisic acid (ABA) content and level of some cytokinins in plant material by ELISA (Enzyme-Linked ImmunoSorbent Assay)

Screening methods – evaluation of resistance level against abiotic stresses of genotypes and/or cultivars of crop plants on the basis of chlorophyll a fluorescence measurement and status of stomata using precision monitoring of testing conditions like temperature, CO2 concentration, air humidity and soil water content

Determination of total antioxidant activity - measurement of low-molecular antioxidants in plant tissues by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method adopted to microtitration plates using common plate reader


PUBLICATIONS

Publications with citations and indexes available on the following websites:


Selected publications listed by year with links to the publishers' websites:

Żur I., Dubas E., Krzewska M., Zieliński K., Fodor J. & Janowiak F. (2019) Glutathione provides antioxidative defence and promotes microspore-derived embryo development in isolated microspore cultures of triticale (× Triticosecale Wittm.). Plant Cell Reports, 38, 195-209. Read more.

Gołębiowska-Pikania G., Kopeć P., Surówka E., Janowiak F., Krzewska M., Dubas E., Nowicka A., Kasprzyk J., Ostrowska A., Malaga S., Hura T. & Żur I. (2017) Changes in protein abundance and activity induced by drought during generative development of winter barley (Hordeum vulgare L.). Journal of Proteomics, 169, 73-86. Read more.

Jurczyk B., Pociecha E., Janowiak F., Kabała D. & Rapacz M. (2016) Variation in waterlogging-triggered stomatal behavior contributes to changes in the cold acclimation process in prehardened Lolium perenne and Festuca pratensis. Plant Physiology and Biochemistry, 109, 280-292. Read more.

Grzesiak M.T., Janowiak F., Szczyrek P., Kaczanowska K., Ostrowska A., Rut R., Hura T., Rzepka A. & Grzesiak S. (2016) Impact of soil compaction stress combined with drought or waterlogging on the physiological and biochemical markers in two maize hybrids. Acta Physiologiae Plantarum, 38, 1-15. Read more.

Maślanka M., Bach A. & Janowiak F. (2016) Endogenous ABA content in relation to maturation of somatic embryos in Tulipa (L.) ‘Apeldoorn’ cultures. Acta Physiologiae Plantarum, 38, 270. Read more.

Eckstein A., Krzeszowiec W., Banaś A.K., Janowiak F. & Gabryś H. (2016) Abscisic acid and blue light signaling pathways in chloroplast movements in Arabidopsis mesophyll. Acta Biochimica Polonica, 63, 449–458. Read more.

Rutowicz K., Puzio M., Halibart-Puzio J., Lirski M., Kotliński M., Kroteń M.A., Knizewski L., Lange B., Muszewska A., Śniegowska-Świerk K., Kościelniak J., Iwanicka-Nowicka R., Buza K., Janowiak F., Żmuda K., Jõesaar I., Laskowska-Kaszub K., Fogtman A., Kollist H., Zielenkiewicz P., Tiuryn J., Siedlecki P., Swiezewski S., Ginalski K., Koblowska M., Archacki R., Wilczynski B., Rapacz M. & Jerzmanowski A. (2015) A Specialized Histone H1 Variant Is Required for Adaptive Responses to Complex Abiotic Stress and Related DNA Methylation in Arabidopsis. Plant Physiology, 169, 2080-2101. Read more.

Żur I., Dubas E., Krzewska M. & Janowiak F. (2015) Current insights into hormonal regulation of microspore embryogenesis. Frontiers in Plant Science, 6, 424. Read more.

Żur I., Dubas E., Krzewska M., Waligórski P., Dziurka M. & Janowiak F. (2015) Hormonal requirements for effective induction of microspore embryogenesis in triticale (× Triticosecale Wittm.) anther cultures. Plant Cell Reports, 34, 47-62. Read more.

Grzesiak M., Ostrowska A., Hura K., Rut G., Janowiak F., Rzepka A., Hura T. & Grzesiak S. (2014) Interspecific differences in root architecture among maize and triticale genotypes grown under drought, waterlogging and soil compaction. Acta Physiologiae Plantarum, 36, 3249-3261. Read more.

Żur I., Dubas E., Krzewska M., Janowiak F., Hura K., Pociecha E., Bączek-Kwinta R. & Płażek A. (2014) Antioxidant activity and ROS tolerance in triticale (× Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis. Plant Cell, Tissue and Organ Culture (PCTOC), 119, 79-94. Read more.

Dubas E., Janowiak F., Krzewska M., Hura T. & Żur I. (2013) Endogenous ABA concentration and cytoplasmic membrane fluidity in microspores of oilseed rape (Brassica napus L.) genotypes differing in responsiveness to androgenesis induction. Plant Cell Reports, 32, 1465-1475. Read more.

Grzesiak M., T, Waligórski P., Janowiak F., Marcińska I., Hura K., Szczyrek P. & Głąb T. (2013) The relations between drought susceptibility index based on grain yield (DSIGY) and key physiological seedling traits in maize and triticale genotypes. Acta Physiologiae Plantarum, 35, 549-565. Read more.

Marcińska I., Czyczyło-Mysza I., Skrzypek E., Grzesiak M., Janowiak F., Filek M., Dziurka M., Dziurka K., Waligórski P., Juzoń K., Cyganek K. & Grzesiak S. (2013) Alleviation of osmotic stress effects by exogenous application of salicylic or abscisic acid on wheat seedlings. International Journal of Molecular Sciences, 14, 13171-13193. Read more.

Marcińska I., Czyczyło-Mysza I., Skrzypek E., Filek M., Grzesiak S., Grzesiak M.T., Janowiak F., Hura T., Dziurka M., Dziurka K., Nowakowska A. & Quarrie S. (2013) Impact of osmotic stress on physiological and biochemical characteristics in drought-susceptible and drought-resistant wheat genotypes. Acta Physiologiae Plantarum, 35, 451-461. Read more.

Pociecha E., Płażek A., Janowiak F., Dubert F., Kolasińska I. & Irla M. (2013) Factors contributing to enhanced pink snow mould resistance of winter rye (Secale cereale L.) – Pivotal role of crowns. Physiological and Molecular Plant Pathology, 81, 54-63. Read more.

Pociecha E., Janowiak F., Dubas E., Żur I., Tokarz K., Kolasińska I. & Płażek A. (2013) Progress of snow mould infection in crowns of winter rye (Secale cereale L.) is related to photosynthetic activity during cold acclimation. Plant Physiology and Biochemistry, 70, 360-367. Read more.

Grzesiak M.T., Marcińska I., Janowiak F., Rzepka A. & Hura T. (2012) The relationship between seedling growth and grain yield under drought conditions in maize and triticale genotypes. Acta Physiologiae Plantarum, 34, 1757-1764. Read more.

Wójcik-Jagła M., Rapacz M., Barcik W. & Janowiak F. (2012) Differential regulation of barley (Hordeum distichon) HVA1 and SRG6 transcript accumulation during the induction of soil and leaf water deficit. Acta Physiologiae Plantarum, 34, 2069-2078. Read more.

Żur I., Krzewska M., Dubas E., Gołębiowska-Pikania G., Janowiak F. & Stojałowski S. (2012) Molecular mapping of loci associated with abscisic acid accumulation in triticale (× Triticosecale Wittm.) anthers in response to low temperature stress inducing androgenic development. Plant Growth Regulation, 1-10. Read more.

Płażek A., Dubert F., Janowiak F., Krępski T. & Tatrzańska M. (2011) Plant age and in vitro or in vivo propagation considerably affect cold tolerance of Miscanthus x giganteus. European Journal of Agronomy, 34, 163-171. Read more.

Płażek A., Dubert F., Pociecha E., Janowiak F., Kolasińska I. & Maciejewski M. (2011) Resistance of winter rye (Secale cereale L.) to Microdochium nivale depends on soluble carbohydrate content but not on abscisic acid level. Journal of Phytopathology, 159, 751-758. Read more.

Jensen C.R., Battilani A., Plauborg F., Psarras G., Chartzoulakis K., Janowiak F., Stikic R., Jovanovic Z., Li G.T., Qi X.B., Liu F.L., Jacobsen S.E. & Andersen M.N. (2010) Deficit irrigation based on drought tolerance and root signalling in potatoes and tomatoes. Agricultural Water Management, 98, 403-413. Read more.

Else M.A., Janowiak F., Atkinson C.J. & Jackson M.B. (2009) Root signals and stomatal closure in relation to photosynthesis, chlorophyll a fluorescence and adventitious rooting of flooded tomato plants. Annals of Botany, 103, 313-323. Read more.

Kalemba E.M., Janowiak F. & Pukacka S. (2009) Desiccation tolerance acquisition in developing beech (Fagus sylvatica L.) seeds: the contribution of dehydrin-like protein. Trees - Structure and Function, 23, 305-315. Read more.

Pociecha E., Płażek A., Janowiak F. & Zwierzykowski Z. (2009) ABA level, proline and phenolic concentration, and PAL activity induced during cold acclimation in androgenic Festulolium forms with contrasting resistance to frost and pink snow mould (Microdochium nivale). Physiological and Molecular Plant Pathology, 73, 126-132. Read more.

Pociecha E., Płażek A., Janowiak F., Waligórski P. & Zwierzykowski Z. (2009) Changes in abscisic acid, salicylic acid and phenylpropanoid concentrations during cold acclimation of androgenic forms of Festulolium (Festuca pratensis x Lolium multiflorum) in relation to resistance to pink snow mould (Microdochium nivale). Plant Breeding, 128, 397-403. Read more.

Liu F., Shahnazari A., Jacobsen S.-E., Jensen C.R., Janowiak F., Waligórski P. & Andersen M.N. (2008) Effects of deficit irrigation and partial root-zone drying on soil and plant water status, stomatal conductance, plant growth and water use efficiency in tomato during early fruiting stage. In: Acta Horticulturae 792, ISHS 2008, Proceedings of the Vth International Symposium on Irrigation of Horticultural Crops (eds I. Goodwin & M.G. O'Connell), pp. 413-419. Read more.

Pociecha E., Płażek A., Janowiak F., Janeczko A. & Zwierzykowski Z. (2008) Physiological basis for differences in resistance to Microdochium nivale (Fr.) Samuels and Hallett in two androgenic genotypes of Festulolium derived from tetraploid F1 hybrids of Festuca pratensis x Lolium multiflorum (Festulolium). Journal of Phytopathology, 156, 155-163. Read more.

Żur I., Dubas E., Golemiec E., Szechyńska-Hebda M., Janowiak F. & Wędzony M. (2008) Stress-induced changes important for effective androgenic induction in isolated microspore culture of triticale (xTriticosecale Wittm.). Plant Cell Tissue and Organ Culture, 94, 319-328. Read more.

Kościelniak J., Janowiak F. & Kurczych Z. (2005) Increase in photosynthesis of maize hybrids (Zea mays L.) at suboptimal temperature (15°C) by selection of parental lines on the basis of chlorophyll alpha fluorescence measurements. Photosynthetica, 43, 125-134. Read more.

Jackson M.B., Saker L.R., Crisp C.M., Else M.A. & Janowiak F. (2003) Ionic and pH signalling from roots to shoots of flooded tomato plants in relation to stomatal closure. Plant and Soil, 253, 103-113. Read more.

Janowiak F., Luck E. & Dörffling K. (2003) Chilling tolerance of maize seedlings in the field during cold periods in spring is related to chilling-induced increase in abscisic acid level. Journal of Agronomy and Crop Science, 189, 156-161. Read more.

Rapacz M., Waligórski P. & Janowiak F. (2003) ABA and gibberellin-like substances during prehardening, cold acclimation, de- and reacclimation of oilseed rape. Acta Physiologiae Plantarum, 25, 151-161. Read more.

Janowiak F., Maas B. & Dörffling K. (2002) Importance of abscisic acid for chilling tolerance of maize seedlings. Journal of Plant Physiology, 159, 635-643. Read more.

Rapacz M., Tokarz K. & Janowiak F. (2001) The initiation of elongation growth during long-term low- temperature stay of spring-type oilseed rape may trigger loss of frost resistance and changes in photosynthetic apparatus. Plant Science, 161, 221-230. Read more.

Rapacz M. & Janowiak F. (1999) Relationship between prehardening, photosynthetic activity at cold acclimation temperatures and frost tolerance in winter rape (Brassica napus var. oleifera). The consequences for the reliability of frost resistance estimation under controlled conditions. Journal of Agronomy and Crop Science, 182, 57-63. Read more.

Rapacz M. & Janowiak F. (1998) Physiological effects of winter rape (Brassica napus var. oleifera) prehardening to frost. I. Frost resistance and photosynthesis during cold acclimation. Journal of Agronomy and Crop Science, 181, 13-20. Read more.

Janowiak F. & Dörffling K. (1996) Chilling of maize seedlings: Changes in water status and abscisic acid content in ten genotypes differing in chilling tolerance. Journal of Plant Physiology, 147, 582-588. Read more.

Janowiak F. & Dörffling K. (1996) Chilling tolerance of 10 maize genotypes as related to chilling-induced changes in ACC and MACC contents. Journal of Agronomy and Crop Science, 177, 175-184. Read more.

Janowiak F. & Dörffling K. (1995) Chilling-induced changes in the contents of 1- aminocyclopropane-1-carboxylic acid (ACC) and its N-malonyl conjugate (MACC) in seedlings of two maize inbreds differing in chilling tolerance. Journal of Plant Physiology, 147, 257-262. Read more.

Janowiak F. & Markowski A. (1994) Changes in leaf water relations and injuries in maize seedlings induced by different chilling conditions. Journal of Agronomy and Crop Science, 172, 19-28. Read more.

Kościelniak J., Janowiak F. & Biesaga-Kościelniak J. (1993) Effect of low soil temperature on weight increase, gas exchange and distribution of 14C-assimilates in seedlings of a maize hybrid. Journal of Agronomy and Crop Science, 170, 163-170. Read more.

Markowski A., Augustyniak G. & Janowiak F. (1990) Sensitivity of different species of field crops to chilling temperature Part III. ATP content and electrolyte leakage from seedling leaves. Acta Physiologiae Plantarum, 12, 167-173.

Janowiak F. (1989) Effect of water saturated atmosphere on chilling injuries of maize seedlings (Zea mays L.). Acta Physiologiae Plantarum, 11, 89-96.

Janowiak F. & Markowski A. (1987) Effect of chilling on germination, growth, survival and membrane permeability in seedling of different forms of maize (Zea mays L.). Acta Physiologiae Plantarum, 9, 77-87.

A searchable list of publications is also available at IPP PAS database.


RECENT POSTERS

Żur I., Surówka E., Dubas E., Nowicka A., Krzewska M., Hura T., Rapacz M., Wójcik-Jagła M., Ostrowska A., Malaga S. & Janowiak F. Antioxidative system activity as the marker for freezing and drought tolerance in winter barley (Hordeum vulgare L.). Poster at the 4th Conference of Cereal Biotechnology and Breeding, November 6-9, 2017, Budapest, Hungary. Book of Abstracts, p. 103-104.

Kaczanowska K., Janowiak F., Jing H.-C., Bekele W.A., Samans B. & Snowdon R.J. Abscisic acid level and osmotic adjustment of sorghum roots under chilling conditions. Poster N28 at Plant Abiotic Stress Tolerance III, June 29 – July 1, 2015, Vienna, Austria. Program and Abstracts, p. 47.

Janowiak F., Kaczanowska K., Jing H.-C., Bekele W.A. & Snowdon R.J. Metabolic limitations to photosynthetic efficiency of sorghum seedling leaves at low temperature. Poster at Agriculture and Climate Change: Adapting Crops to Increased Uncertainty, February 15-17, 2015, Amsterdam, the Netherlands. Procedia Environmental Sciences 29, 277-278.

Żur I., Dubas E., Krzewska M., Nowicka A., Dziurka M., Waligórski P. & Janowiak F. Determinants of effective microspore embryogenesis in triticale (× Triticosecale Wittm.), Poster at 3rd Conference of Cereal Biotechnology and Breeding CBB3, November 2-4, 2015, Berlin, Germany.

Grzesiak M., Janowiak F., Szczyrek P., Kaczanowska K., Ostrowska A., Rut G., Rzepka A. & Grzesiak S. Multistress effect of soil compaction combined with drought or waterlogging on changes in physiological characteristics in maize (Zea mays L.) hybrids. Poster No. PPS002 at Botany 2015, July 25-29, 2015, Edmonton, Alberta, Canada.

Żur I., Dubas E., Krzewska M. & Janowiak F. Mapping of quantitative trait loci determining antioxidant activity in triticale (× Triticosecale Wittm.) anthers associated with androgenesis initiation. Poster at The quest for tolerant varieties – Phenotyping at plant and cellular level, June 22-24, 2015, IPK Gatersleben, Germany.

Janowiak F., Kaczanowska K., Jing H.-C., Bekele W.A. & Snowdon R.J. Osmotic adjustment, abscisic acid accumulation, and a decrease in photosynthetic efficiency are the key consequences of low temperature influence on sorghum seedlings. Poster at 10th International Plant Cold Hardiness Seminar Stress Recognition Triggers Plant Adaptation, Kórnik-Poznań, Poland, August 17-21, 2014. Book of Abstracts, p. S5.29.

Żur I., Dubas E., Krzewska M., Janowiak F. & Hura K. The role of the antioxidative system in the induction of microspore embryogenesis in anther culture of triticale (× Triticosecale Wittm.). Poster at Plant Symposium of the SEB Oxidative stress and cell death in plants: mechanisms and implications, June 26-28, 2013, Florence, Italy. Book of Abstracts, p.39.

Żur I., Dubas E., Krzewska M., Janowiak F., Waligórski P. & Dziurka M. Modifications in endogenous plant growth regulators level in winter triticale (× Triticosecale Wittm.) anthers associated with induction of microspore embryogenesis. Poster at VIPCAII Plant Genetics and Breeding Technologies, February 18–20, 2013, Vienna, Austria. Book of Abstracts, p.76.


COOPERATION WITH FOREIGN SCIENTIFIC INSTITUTIONS

Institute of General Botany (now Biocenter Klein Flottbek), University of Hamburg – cooperation with Prof. Karl Dörffling in the years 1988-2006 in the form of bilateral projects and research stays as DAAD, DFG and EU (Copernicus, COST 814) fellowship holder

School of Biological Sciences, University of Bristol – cooperation with Prof. Michael B. Jackson in the years 1998-2010 in the form of research project and research visits financed by Royal Society, London

East Malling Research – cooperation with Dr Mark A. Else in the years 2000-2005 in the form of research project and research visits financed by Royal Society, London

CRA Genomic Research Centre of Fiorenzuola d'Arda – cooperation with Prof. Luigi Cattivelli and Dr Fulvia Rizza in the years 2003-2006 in the form of bilateral research project

Institute of Crop Science and Resource Conservation, University of Bonn – cooperation with Prof. Folkard Asch in the years 2004-2007 in the form of research stays as DAAD and DFG fellowship holder

Institute of Plant Production and Agroecology in the Tropics and Subtropics, University of Hohenheim – cooperation with Prof. Folkard Asch since 2007 in the form of Memorandum of Understanding

Department of Agriculture and Ecology, University of Copenhagen – cooperation with Prof. Christian R. Jensen and Prof. Fulai Liu since 2000 in the form of research stays within the exchange programme Cirrius and EU project SAFIR

Department of Plant Breeding, Institute of Agronomy and Plant Breeding, Justus Liebig University Giessen – cooperation with Prof. Rod Snowdon since 2013 in the form of multilateral scientific project ERANET GAS


TEACHING EXPERIENCE

Supervisor of PhD theses at Doctoral Studies in Natural Sciences at the Polish Academy of Sciences in Cracow:

Physiological reactions of tomato plants (Lycopersicon esculentum Mill.) to common and alternating deficit irrigation (Dr Barbara Wojciechowska, 2011)

Physiological reactions of maize (Zea mays L.) and tomato (Lycopersicon esculentum Mill.) plants to abiotic stress factors with special emphasis on root-to-shoot communication (Dr Joanna Sawa, 2011)

Mechanisms of chilling tolerance in sorghum (Sorghum bicolor (L.) Moench) (M.Sc. Katarzyna Kaczanowska, in progress)

Reviewer of PhD theses:

Physiological, ultra-structural and molecular aspects of the inhibition of transport processes in maize leaves under chilling conditions (Dr Anna Bilska, 2007), Plant Breeding and Acclimatization Institute (IHAR) at Radzikow near Warsaw

Stress tolerance and hardiness of woody plants (Dr Majken Pagter, 2008), Faculty of Life Sciences, University of Copenhagen

Physiological basis of Centaurea cyanus L. resistance to tribenuron methyl and usefulness physical and biochemical methods to assess this resistance (Dr Diana Saja, 2013), Pedagogical University in Kraków

Usefulness of fast kinetics measurements of chlorophyll fluorescence for the evaluation of frost tolerance in triticale and wheat (Dr Monika Sasnal, 2014), University of Agriculture in Kraków

Proline accumulation and the activity of pyrroline-5-carboxylate synthetase under drought conditions in two spring barley (Hordeum vulgare) genotypes and homozygous lines originating from their hybridization (Dr Justyna Niedziela, 2017), University of Life Sciences in Poznań