Department of Cell Biology
address: Niezapominajek 21, 30-239 Kraków
telephone: (+48) 12 4253301 (wew./ext. 40)
faks: (+48) 12 4251844
i.zur@ifr-pan.edu.pl
Staff
Scientific Staff
PHD Students
Description
Poster overview of the Department of Cell Biology
RESEARCH TOPICS
Androgenesis in in vitro cultures of cereals, grasses and Brassicaceae – doubled haploid (DH) technology and utilisation of DHs in basic research and breeding practice
Molecular, proteomic, metabolomic and cytological background of androgenesis in hexaploid triticale (× Triticosecale Wittm.) and oilseed rape (Brassica napus L.)
In vitro cultures (tissue/suspension/protoplast) and its utilization in studies on tolerance/resistance and defence reactions in response to (a)biotic stress
Molecular and physiological determinants controlling resistance of winter triticale (× Triticosecale Wittm.) towards Microdochium nivale and Blumeria graminis infection
Epigenetic factors that influence on the process of microspore embryogenesis
Embryology of cassava (Manihot aesculenta Kranz)
RESEARCH COOPERATION OFFER
- Doubled haploids production by androgenesis (anther culture, isolated microspor culture) and distant crosses for basic reasearch (genetic mapping, QTLs analysis, proteomics, metabolomics) and breeding (cooperation with commeracial breeding companies)
- In vitro culture of organs/tissues/cell and protoplasts suspensions and its utilization in the studies on embryo- or organogenesis and defences reactions induced by abiotic stress treatment
- Plant-pathogen interactions, identification of factors that determine the resistance against pathogenic fungi
- Early phases of plant embryogenesis
- Advanced analytic methods of light and fluorescent microscopy: in situ immunodetection, histochemistry, cytogenetics, cariology, cytology and classic anatomy
- Biochemical and molecular analyses: proteins extraction and identyfication (D-Gel, Western-Blot, SELDI-TOF), DNA isolation and analysis (PAGE
Publications
-
- Sychta K.
- Dubas E.
- Yamada K.
- Słomka A.
- Krzewska M.
- Kuta E.
- Papain-like cysteine proteases are involved in programmed cell death in plants under heavy metal stress.
- 2020.
- Environmental and Experimental Botany 174: 104041.
-
- Wójcik-Jagła M.
- Rapacz M.
- Dubas E.
- Krzewska M.
- Kopeć P.
- Nowicka A.
- Ostrowska A.
- Malaga S.
- Żur I.
- Candidate genes for freezing and drought tolerance selected on the basis of proteome analysis in doubled haploid lines of barley..
- 2020.
- International Journal of Molecular Sciences 21: 2062.
-
- Zieliński K.
- Krzewska M.
- Żur I.
- Juzoń K.
- Kopeć P.
- Nowicka A.
- Moravčiková J.
- Skrzypek E.
- Dubas E.
- The effect of glutathione and mannitol on androgenesis in anther and isolated microspore cultures of rye (Secale cereale L.).
- 2020.
- Plant Cell, Tissue and Organ Culture 140: 577–592 .
-
- Nowicka A.
- Tokarz B.
- Zwyrtková J.
- Dvořák-Tomaštíková E.
- Procházková K.
- Ercan U.
- Finke A.
- Poppenberger B.
- Otmar M.
- Niezgodzki I.
- Krečmerová M.
- Schubert I.
- Pecinka A.
- Comparative analysis of epigenetic inhibitors reveals different degrees of interference with transcriptional gene silencing and induction of DNA damage..
- 2020.
- The Plant Journal,102:68–84..
Projects
-
Tango2/340285/NCBR/2017,
Protocol for effective doubled haploids production by androgenesis for polish breeding materials of wheat (Triticum aestivum L.) -
2015/18/M/NZ3/00348,
Oxidative stress and its role in the regulation of embryogenesis in isolated microspore cultures of triticale (x Triticale Wittm.) and barley (Hordeum vulgare L.) -
MRiRW, nr 84,
Identification of factors determining the efficiency of doubled haploids production in rye (Secale cereale L.) through androgenesis and distant crosses -
MRiRW, nr 26,
Identification of factors which determine drought and freezing tolerance in barley (Hordeum vulgare L.)