Department of Cell Biology
address: Niezapominajek 21, 30-239 Kraków
telephone: (+48) 12 4253301 (wew./ext. 40)
faks: (+48) 12 4251844
i.zur@ifr-pan.edu.pl
Staff
Scientific Staff
PHD Students
Description
Poster overview of the Department of Cell Biology
RESEARCH TOPICS
Androgenesis in in vitro cultures of cereals, grasses and Brassicaceae – doubled haploid (DH) technology and utilisation of DHs in basic research and breeding practice
Molecular, proteomic, metabolomic and cytological background of androgenesis in hexaploid triticale (× Triticosecale Wittm.) and oilseed rape (Brassica napus L.)
In vitro cultures (tissue/suspension/protoplast) and its utilization in studies on tolerance/resistance and defence reactions in response to (a)biotic stress
Molecular and physiological determinants controlling resistance of winter triticale (× Triticosecale Wittm.) towards Microdochium nivale and Blumeria graminis infection
Epigenetic factors that influence on the process of microspore embryogenesis
Embryology of cassava (Manihot aesculenta Kranz)
RESEARCH COOPERATION OFFER
- Doubled haploids production by androgenesis (anther culture, isolated microspor culture) and distant crosses for basic reasearch (genetic mapping, QTLs analysis, proteomics, metabolomics) and breeding (cooperation with commeracial breeding companies)
- In vitro culture of organs/tissues/cell and protoplasts suspensions and its utilization in the studies on embryo- or organogenesis and defences reactions induced by abiotic stress treatment
- Plant-pathogen interactions, identification of factors that determine the resistance against pathogenic fungi
- Early phases of plant embryogenesis
- Advanced analytic methods of light and fluorescent microscopy: in situ immunodetection, histochemistry, cytogenetics, cariology, cytology and classic anatomy
- Biochemical and molecular analyses: proteins extraction and identyfication (D-Gel, Western-Blot, SELDI-TOF), DNA isolation and analysis (PAGE
Publications
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- Wójcik-Jagła M.
- Daszkowska-Golec A.
- Fiust A.
- Kopeć P.
- Rapacz M.
- Identification of the genetic basis of response to de-acclimation in winter barley.
- 2021.
- International Journal of Molecular Sciences, 22: 1057.
-
- Hornyák M.
- Słomka A.
- Sychta K.
- Dziurka M.
- Kopeć P.
- Pastuszak J.
- Szczerba A.
- Płażek A.
- Reducing flower competition for assimilates by half results in higher yield of Fagopyrum esculentum.
- 2020.
- International Journal of Molecular Sciences, 21: 8953.
-
- Surówka E.
- Hura T.
- Osmoprotectants and nonenzymatic antioxidants in halophytes.
- 2020.
- In: Handbook of Halophytes. From Molecules to Ecosystems towards Biosaline Agriculture. Grigore MN. (eds); publisher name: Springer, Cham.
-
- Kovacik M.
- Nowicka A.
- Pecinka A
- Isolation of high purity tissues from developing barley seeds..
- 2020.
- Journal of Visualized Experiments, 164: e61681.
Projects
-
MRiRW nr 4,
Identyfikacja czynników warunkujących indukcję embriogenezy mikrospor u pszenicy zwyczajnej (Triticum aestivum L.) -
Tango2/340285/NCBR/2017,
Protocol for effective doubled haploids production by androgenesis for polish breeding materials of wheat (Triticum aestivum L.) -
2015/18/M/NZ3/00348,
Oxidative stress and its role in the regulation of embryogenesis in isolated microspore cultures of triticale (x Triticale Wittm.) and barley (Hordeum vulgare L.) -
MRiRW, nr 84,
Identification of factors determining the efficiency of doubled haploids production in rye (Secale cereale L.) through androgenesis and distant crosses