Department of Cell Biology
address: Niezapominajek 21, 30-239 Kraków
telephone: (+48) 12 4253301 (wew./ext. 40)
faks: (+48) 12 4251844
i.zur@ifr-pan.edu.pl
Staff
Scientific Staff
PHD Students
Description
Poster overview of the Department of Cell Biology
RESEARCH TOPICS
Androgenesis in in vitro cultures of cereals, grasses and Brassicaceae – doubled haploid (DH) technology and utilisation of DHs in basic research and breeding practice
Molecular, proteomic, metabolomic and cytological background of androgenesis in hexaploid triticale (× Triticosecale Wittm.) and oilseed rape (Brassica napus L.)
In vitro cultures (tissue/suspension/protoplast) and its utilization in studies on tolerance/resistance and defence reactions in response to (a)biotic stress
Molecular and physiological determinants controlling resistance of winter triticale (× Triticosecale Wittm.) towards Microdochium nivale and Blumeria graminis infection
Epigenetic factors that influence on the process of microspore embryogenesis
Embryology of cassava (Manihot aesculenta Kranz)
RESEARCH COOPERATION OFFER
- Doubled haploids production by androgenesis (anther culture, isolated microspor culture) and distant crosses for basic reasearch (genetic mapping, QTLs analysis, proteomics, metabolomics) and breeding (cooperation with commeracial breeding companies)
- In vitro culture of organs/tissues/cell and protoplasts suspensions and its utilization in the studies on embryo- or organogenesis and defences reactions induced by abiotic stress treatment
- Plant-pathogen interactions, identification of factors that determine the resistance against pathogenic fungi
- Early phases of plant embryogenesis
- Advanced analytic methods of light and fluorescent microscopy: in situ immunodetection, histochemistry, cytogenetics, cariology, cytology and classic anatomy
- Biochemical and molecular analyses: proteins extraction and identyfication (D-Gel, Western-Blot, SELDI-TOF), DNA isolation and analysis (PAGE
Publications
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- Kopeć P.
- Hornyák M.
- Pastuszak J.
- Szczerba A.
- Rapacz M.
- Waga J.
- Płażek A.
- Changes in the Flower and Leaf Proteome of Common Buckwheat (Fagopyrum esculentum Moench) under High Temperature .
- 2021.
- International Journal of Molecular Sciences. 22/5: 2678.
-
- Makowski W.
- Królicka A.
- Nowicka A.
- Zwyrtková J.
- Tokarz B.
- Pecinka A.
- Banasiuk R.
- Tokarz K.
- Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties.
- 2021.
- Applied Microbiology and Biotechnology, 105: 1215–1226 .
-
- Nowicka A.
- Kovacik M.
- Tokarz B.
- Vrána J.
- Zhang Y.
- Weigt D.
- Doležel J.
- Pecinka A.
- Dynamics of endoreduplication in developing barley seeds.
- 2021.
- Journal of Experimental Botany, 72: 268–282.
-
- Zieliński K.
- Dubas E.
- Gerši Z.
- Krzewska M.
- Janas A.
- Nowicka A.
- Matušíková I.
- Żur I.
- Sakuda S.
- Moravčíková J.
- β-1,3-Glucanases and chitinases participate in the stress-related defence mechanisms that are possibly connected with modulation of arabinogalactan proteins (AGP) required for the androgenesis initiation in rye (Secale cereale L.).
- 2021.
- Plant Science, 302: 110700.
Projects
-
MRiRW nr 4,
Identyfikacja czynników warunkujących indukcję embriogenezy mikrospor u pszenicy zwyczajnej (Triticum aestivum L.) -
Tango2/340285/NCBR/2017,
Protocol for effective doubled haploids production by androgenesis for polish breeding materials of wheat (Triticum aestivum L.) -
2015/18/M/NZ3/00348,
Oxidative stress and its role in the regulation of embryogenesis in isolated microspore cultures of triticale (x Triticale Wittm.) and barley (Hordeum vulgare L.) -
MRiRW, nr 84,
Identification of factors determining the efficiency of doubled haploids production in rye (Secale cereale L.) through androgenesis and distant crosses