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Dr hab Ewa Dubas

Associate Professors
Department of Cell Biology
(+48) 12 4253301 (wew./ext. 39)
e.dubas@ifr-pan.edu.pl
The mechanisms of gametogenesis, non-zygotic embryogenesis (androgenesis), and embryo formation (cytoskeleton, endogenous hormonal- and (epi)genetic control, embryo polarity, programmed cell death) in crops • double haploids • pathogenesis

Publications

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Projects

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DIPLOMAS, DEGREES

2015- present Associate Professor in Agriculture, Agriculture University, Kraków, Poland
2007- PhD in Biology, Jagiellonian University, Kraków, Poland
2001- M.Sc in Biology – Department of Plant Cytology and Embryology, Jagiellonian University, Krakow, Poland

EMPLOYMENT IN ACADEMIC INSTITUTIONS

2020-present

Associate Professor, Department of Cell Biology in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow

2020-2020

Adiunct [Assistant Professor], Department of Plant Cytology and Embryology, Jagiellonian University, Krakow

2015-2019

Associate Professor, Department of Cell Biology in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow

2007-2015

Adiunct [Assistant Professor], Department of Cell Biology in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow

2001-2007

PhD student, Department of Cell Biology in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow

2001-2007

Interdisciplinary Ph.D. Study in the Faculty of Biology and Earth Sciences, Jagiellonian University

MEMBERSHIP AND FUNCTIONS

2020-now Member of the Scientific Council in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow
2019-2020 Member of the Scientific Council of the Faculty of Biology of the Jagiellonian University in Krakow 
2015-2019 Member of the Scientific Council in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow
2014-2017 Polish deputy in Management Committee of COST FA1306 ‘The quest for tolerant varieties: 'Phenotyping at plant and cellular level’
2014 External expert evaluator of the international research projects Fund for Scientific Research – FNRS; Slovak Research and Development Agency; Czech National Agency
2011-2013 Polish deputy in Management Committee of COST FA (Food and Agriculture) 0903 ‘Harnessing Plant Reproduction for Crop Improvement’

RESEARCH AREAS

  • Cell biology (cytoskeleton, cell cycle, auxin and abscisic acid signaling, plasma membrane fluidity, cell death) in responses to abiotic and biotic stress.

  • Effect of stress on plant’s responses in gene and protein levels, transgenesis, plant-growth regulators, in vitro tissue culture of cereals and Brassicaceae.

  • Pathogenesis - cytological and physiological analyses of the infection processes.

PRACTICAL SKILLS

  • In vitro culture: oil-seed rape microspore culture, cereal anther and microspore cultures, immature embryo culture.

  • Tracing of molecules in plant material: analysis of alfa-tubulin, actin, auxin, and auxin efflux carrier proteins localization by immunocytochemistry, fluorescent and confocal laser scanning microscopy (CLSM).

  • Modeling of cellular processes: Graphical Visualization of Cellular Processes in Image Pro-Premier

  • Tracing of the fungal infection process in plant material by cytology and microscopy.

  • Protein analyses: immunolocalisation of proteins in intact cells ('whole mount') and in sectioned material, protein isolation electrophoresis and western blotting.

  • Gene expression: RNA, DNA and pDNA isolation, PCR, qPCR, primer design.

  • Restriction analysis.

  • Bioinformatics: Sequence analysis, gene, and protein sequence alignment: BLAST.

  • Chromosomes analyses: karyotype analyses, C-banding of chromosomes.

  • Quantitative and qualitative analysis of ROS and antioxidative enzyme activity:  fluorescence and fluorometric measurements.

  • Microspores/Androgenic embryos transformation with Agrobacterium tumefaciens.

RESEARCH EXPERIENCE

Post doc projects. My Post Doc projects concentrated mainly on: (1) endogenous auxins as a triggers in double haploids (DHs) production in oilseed rape, (2) the role of polar auxin transport and auxin signalling in embryo development. Plant embryonic development involves differential distribution (gradients) of the plant signaling molecule auxin. These gradients are established and maintained by a directional, intercellular auxin transport called polar auxin transport. Polar auxin transport provides positional and directional information for many aspects of embryo development. B. napus microspore suspension is a suitable model system for such studies. By using combination of genetic, molecular, physiological and cell biology approaches I would like to address the key question: how plant hormones perform their respective roles in embryo development, with a special focus on hormonal interactions.

 

PhD project. My PhD project concentrated on early embryo development in microspore suspension in vitro cultures of Brassica napus. High yields of microspore-derived embryos MDE in suspension were reproducibly obtained according to the method elaborated previously by research team of dr Jan Custers PRI Wageningen, The Netherlands. I adopted the method and further optimised in my laboratory. MDE develop and differentiate in a way resembling zygotic embryos in almost every studied aspect, therefore I used them to study early stages of plant embryogenesis. The main goal of these studies was to study the development of androgenic embryos from the earliest stages (a single cell) onwards including suspensor formation. Studying those structures during zygotic development is very difficult due to ovular tissues that surround embryos. I focused on establishment of embryo polarity and the role of cytoskeleton during MDE differentiation tracing also mitochondria and amyloplasts positions, vacuolisation pattern, cell-wall formation and differentiation of embryo organs. My studies required optimalization of many methods and development of protocols of immunodetection and tracing of various structures in whole-mount (not sectioned) material.

            

 

PUBLICATIONS

                   

 

Selected older papers (up to 2017) listed by year with links to the publishers' websites:

2015-2018

  1. Golebiowska-Pikania G., Kopeć P., Surówka E., Janowiak F., Krzewska M., Dubas E., Nowicka A., Kasprzyk J., Ostrowska A., Malaga S., Hura T., Żur I. 2017. Changes in protein abundance and activity induced by drought during generative development of winter barley (Hordeum vulgare L.). Journal of Proteomics 169:73-86, doi: 10.1016/j.jprot.2017.07.016 Strona wydawcy 
  2. Golebiowska-Pikania G., Kopeć P., Surówka E., Krzewska M., Dubas E., Nowicka A., Rapacz M., Wójcik-Jagła M., Malaga S., Żur I. 2017. Changes in protein abundance and activity involved in freezing tolerance acquisition in winter barley (Hordeum vulgare L.). Journal of Proteomics 169:58-72, doi: 10.1016/j.jprot.2017.08.019 Strona wydawcy
  3. Krzewska M., Gołębiowska-Pikania G., Dubas E., Gawin M., Żur I. 2017. Identification of proteins related to microspore embryogenesis responsiveness in winter triticale (×Triticosecale Wittm.). Euphytica 213:192. doi: 10.1007/s10681-017-1978-1 Strona wydawcy
  4. Śniegowska-Świerk K., Dubas E. & Rapacz M. 2016. Actin microfilaments are involved in the regulation of HVA1 transcript accumulation in drought-treated barley leaves. Journal of Plant Physiology, 193, 22-25. Strona wydawcy
  5. Wędzony M., Żur I., Krzewska M., Dubas E., Szechyńska-Hebda M., Wąsek I. 2015. Doubled haploids in triticale. Eudes F. (ed.), Triticale, Springer, pp.111-128. Strona wydawcy
  6. Krzewska M., Czyczyło-Mysza I., Dubas E., Gołębiowska-Pikania G., Żur I. 2015. Identification of QTLs associated with albino plant formation and some new facts concerning green versus albino ratio determinants in triticale (×Triticosecale Wittm.) anther culture. Euphytica, 1-16. doi: 10.1007/s10681-015-1509-x Strona wydawcy
  7. Żur I., Dubas E., Krzewska M., Waligórski P., Dziurka M., Janowiak F. 2015. Hormonal requirements for effective induction of microspore embryogenesis in triticale (×Triticosecale Wittm.) anther cultures. Plant Cell Rep. 34(1):47-62. Strona wydawcy
  8. Żur I., Dubas E., Krzewska M., Janowiak F. 2015. Current insights into hormonal regulation of microspore embryogenesis. Frontiers in Plant Science 6 doi:10.3389/fpls.2015.00424 Strona wydawcy
  9. Żur I., Dubas E., Krzewska M., Waligórski P.,  Dziurka M.,  Janowiak F. 2015. Hormonal requirements for effective induction of microspore embryogenesis in triticale (× Triticosecale Wittm.) anther cultures. Plant Cell Reports, 34:47-62, doi:10.1007/s00299-014-1686-4 Strona wydawcy

  1. Dubas E., Moravčíková J., Libantová J., Matušíková I., Benkova E., Żur I., Krzewska M. 2014. The influence of heat stress on auxin distribution in transgenic B. napus microspores and microspore derived embryos. Protoplasma 251:1077-1087. IF2013 3.171  Read more

  2. Dubas E., Custers J., Kieft H., Wędzony M., van Lammeren A.A.M. 2014. Characterization of polarity development through 2- and 3-D imaging during the initial phase of microspore embryogenesis in Brassica napus L. Protoplasma 251: 103-113. IF2013 3.171  Read more

  3. Wędzony M., Szechyńska-Hebda M., Żur I., Dubas E., Krzewska M. 2014. Tissue culture and regeneration: a prerequisite for alien gene transfer. In: Alien Gene Transfer in Crop Plants, Vol. 1. Innovations, Methods and Risk Assessment. Pratap A., Kumar, J. (Eds.), Springer, ISBN 978-1-4614-8584-1 Read more

  4. Żur I., Dubas E., Krzewska M., Janowiak F., Hura K., Pociecha E., Bączek-Kwinta R., Płażek A. 2014. Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis. Plant Cell, Tissue and Organ Culture (PCTOC). DOI: 10.1007/s11240-014-0515. IF2013: 2.61  Read more

  5. Żur I.,.Dubas E*., Krzewska M., Sánchez-Díaz R.A., Castillo A.M., Vallés M.P. 2014. Changes in gene expression patterns associated with microspore embryogenesis in hexaploid triticale (× Triticosecale Wittm.)", submitted to Plant Cell, Tissue and Organ Culture (PCTOC) 116(2):261-267. IF2013 2.612  Read more

  6. Żur I.,.Dubas E., Krzewska M., Waligórski P., Dziurka M., Janowiak F. 2014. Hormonal requirements for effective induction of microspore embryogenesis in triticale (×Triticosecale Wittm.) anther cultures. Plant Cell Rep DOI 10.1007/s00299-014-1686-4. MNiSW 35 pkt., IF2013 2,936  Strona wydawcy

  7. Dubas E., Janowiak F., Krzewska M., Hura T., Żur I. 2013. Endogenous ABA concentration and cytoplasmic membrane fluidity in microspores of oilseed rape (Brassica napus L.) genotypes differing in responsiveness to androgenesis induction. Plant Cell Rep 32:1465–1475. IF2013 2.936  Read more

  8. Pociecha E., Janowiak F., Dubas E., Żur I., Tokarz K., Kolasińska I., Płażek A. 2013. Progress of snow mould infection in crowns of winter rye (Secale cereale L.) is related to photosynthetic activity during cold acclimation. Plant Physiology and Biochemistry 70:360-367. IF2013: 2.352 Read more

  9. Żur I., Gołębiowska G., Dubas E., Golemiec E., Matušíková I., Libantová J., Moravčíková J. 2013. β-1,3-glucanase and chitinase activities in winter triticales during cold hardening and subsequent infection by Microdochium nivale. Biologia 68/2: 241-248. IF2013: 0.696 Read more

  10. Żur I., Dubas E., Słomka A., Dubert F., Kuta E., Płażek A. 2013. Failure of androgenesis in Miscanthus×giganteus in vitro culture of cytologically unbalanced microspores. Plant Reproduction 26(3):297-307. IF2012: 2.07   Read more

  1. Dubas E., Wędzony M., Custers J., Kieft H., van Lammeren A.A.M. 2012. Gametophytic development of Brassica napus pollen in vitro enables examination of cytoskeleton and nuclear movements. Protoplasma 249(2): 369-377. IF2013: 3.171  Read more

  2. Krzewska M., Czyczyło-Mysza I., Dubas E., Gołębiowska-Pikania G., Golemiec E., Stojałowski S., Chrupek M., Żur I. 2012. Quantitative trait loci associated with androgenic responsiveness in triticale (×Triticosecale Wittm.) anther culture. Plant Cell Reports 31:2099–2108. Plant Cell Reports 31:2099–2108. IF2013: 2.936  Read more

  3. Słomka A., Kuta E., Płażek A., Dubert F., Żur I., Dubas E., Kopeć P., Żurek G. 2012. Sterility of Miscanthus×giganteus results from hybrid incompatibility. Acta Biologica Cracoviensia Series Botanica 54/1: 1–8. IF2013: 0.662 Read more

  4. Żur I., Krzewska M., Dubas E., Gołębiowska-Pikania G., Janowiak F., Stojałowski S. 2012. Molecular mapping of loci associated with abscisic acid accumulation in triticale (×Triticosecale Wittm.) anthers in response to low temperature stress inducing androgenic development. Plant Growth Regulation 68(3): 483-492. IF2013: 1.625  Read more

  5. Dubas E., Custers J.B.M., Kieft H., Wędzony M., van Lammeren  A.A.M. 2011. Microtubule configurations and nuclear DNA synthesis in microspore derived embryos of Brassica napus cv Topas that mimic zygotic embryo development. Plant Cell Reports 30: 2105–2116. IF2013 : 2.936   Read more

  6. Dubas E., Golebiowska G., Zur I., Wedzony M. 2011. Microdochium nivale (Fr., Samuels & Hallett): cytological analysis of the infection process in triticale (x Triticosecale Wittm.). Acta Physiologiae Plantarum 33(2): 529-537. IF2013: 1.524  Read more

  7. Żur I.A., Dubas E., Pociecha E., Dubert F., Kolasińska I., Płażek A. 2011. Cytological analysis of infection process and the first defence responses induced in winter rye (Secale cereale L.) seedlings inoculated with Microdochium nivale. Physiological and Molecular Plant Pathology 76: 189-196. IF2013: 1.987 Read more

  8. Szechyńska-Hebda M., Wędzony M., Tyrka M., Gołębiowska G., Chrupek M., Czyczyło-Mysza I., Dubas E., Żur I., Golemiec E. 2011. Identifying QTL for cold-induced resistance to Microdochium nivale in winter triticale. Plant Genetic Resources: Characterization and utilization 9(2):296-299. IF2013: 1.057 Read more

  1. Dubas E., Wędzony M., Petrovska B., Salaj J., Żur I. 2010. Cell structural reorganization during induction of androgenesis in isolated microspore cultures of triticale (x Triticosecale Wittm.). Acta Biologica Cracoviensia Series Botanica 52/1: 73–86. IF2013: 0.662 Read more

  2. Dubas E., Marzec K., Płażek A. 2010. Cytological studies on Microdochium nivale infection in Lolium perenne (L.) plants. Zeszyty Problemowe Postępów Nauk Rolniczych PAN Zeszyt 545:389-401.

  3. Żur I., Dubas E., Golemiec E., Szechyńska-Hebda M., Gołębiowska G., Wędzony M. 2009. Stress-related variation in antioxidative enzymes activity and cell metabolism efficiency associated with embryogenesis induction in isolated microspore culture of triticale (xTriticosecale Wittm.). Plant Cell Reports 28:1279–1287. IF2013: 2.36  Read more

  4. Wędzony M., Forster B.P., Żur I., Golemiec E., Szechyńska-Hebda M., Dubas E., Gołębiowska G. 2009.  Progress in Doubled Haploid Technology in Higher Plants. In: Touraev A., Jain M., Forster B. (eds.): “Advances in Haploid Production in Higher Plants” © Springer Science + Business Media B.V., pp.1-35. IC 30 Read more

  1. Supena EDJ., Winarto B., Riksen T., Dubas E., van Lammeren A., Offringa R., Boutilier K., Custers J. 2008. Regeneration of zygotic-like microspore-derived embryos suggests an important role for the suspensor in early embryo patterning. Journal of Experimental Botany 59(4): 803-814. doi:10.1093/jxb/erm358.IF2013: 5.794  Read more

  2. Żur I., Dubas E., Golemiec E., Szechyńska-Hebda M., Janowiak F., Wędzony M. 2008. Stress-induced changes important for effective androgenic induction in isolated microspore culture of triticale (xTriticosecale Wittm.). Plant Cell, Tissue and Organ Culture 94:319-328.  Read more

  3. Szechyńska-Hebda M., Wędzony M., Dubas E., Kieft H., van Lammeren A. 2006. Visualisation of microtubules and actin filaments in fixed BY-2 suspension cells using an optimized whole mount immunolabeling protocol. Plant Cell Reports. 25 (8): 758 – 766. IF2013: 2.936  Read more

  4. Żur I., Godzisz I., Dubas E., Golemiec E., Wędzony M. 2006. The influence of cold pretreatment on androgenesis efficiency in isolated microspore culture of triticale (X Triticosecale Wittm.). Zeszyty Problemowe Postępów Nauk Rolniczych 509: 49-59.

 

COORDINATION/PARTICIPATION IN RESEARCH PROJECTS 

NATIONAL  (RUNNING PROJECTS)

2021 - 2026 ‘Identification of factors determining the efficiency of microspore embryogenesis induction in wheat (Triticum aestivum L.)’ research project financed by Ministry of Agriculture and Rural Development, Task No. No 4; Host Institution: Polish Academy of Sciences The Franciszek Górski Institute of Plant Physiology in Kraków; a role: researcher

NATIONAL  (FINISHED PROJECTS)

2016 - 2021 'Oxidative stress and its role in the regulation of embryogenesis in isolated microspore cultures of triticale (xTriticosecale Wittm.) and barley (Hordeum vulgare L.)' research project financed by National Science Centre; 2015/18/M/NZ3/00348; a role: researcher
2017 - 2020 ‘Protocol for effective doubled haploids production by androgenesis for Polish breeding materials of wheat (Triticum aestivum L.)’ research project financed by National Centre of Research and Development; Tango2/340285/NCBR/2017; a role: researcher
2015 - 2018 ‘Identification of factors determining the efficiency of doubled haploids production in rye (Secale cereale L.) through androgenesis and distant crosses’ research project financed by Ministry of Agriculture and Rural Development, Task No. 84; Host Institution: Polish Academy of Sciences The Franciszek Górski Institute of Plant Physiology in Kraków; a role: project coordinator
2014 - 2018 ‘Identification of factors determine drought and frost resistance in winter barley (Hordeum vulgare L.)’ research project. Biological progress, Task No. 26 (Appendix No. 9 to the Regulation of the Minister of Agriculture and Rural Development on the rate subsidy for various entities performing tasks for agriculture in May 18th, 2010 (Journal of Laws No. 91, Item 595, as amended); a role: researcher
2013 - 2014 ‘Identification of drought induced signalling pathway in barley leaves’. Research national project No. G-1843 ; a role: researcher
2011 - 2014  ‘The role of endogenous auxins: Indole-3-acetic acid (IAA) and Indole-3-butyric acid (IBA) in auxin homeostasis and androgenic effectivness enhancement in oilseed rape (Brassica napus)’. Individual research national project No. 2011/01/D/NZ9/02547; a role: project coordinator

 BILATERAL (FINISHED PROJECTS)

2016 - 2018 ‘Stress-inducible expression of arabinogalactans and defence-related proteins in relation to androgenesis initiation’. Polish - Slovak research project (Host Institutions: IPP PAS and Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences); a role: project coordinator.
2016 - 2018 ‘Chitinases and AGPs as proteins diversifying genotypes with high ability to microspore embryogenesis from non-embryogenic in crops’. CHIAGP, No. SK-PL-2015-0044. Polish – Slovak research project (Host Institutions: IPP PAS and Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences); a role: project coordinator.
2011 - 2014 Auxin as signalling molecule in doubled haploid production of rape (Brassica napus var. oleifera)’. Polish-Belgian bilateral research project (Host Institutions: IPP PAS and VIB in Ghent University); a role: project coordinator.
2011 -2013 ‘N-butanol as a trigger inducing microspore embryogenesis in in vitro cultures of wheat (Triticum aestivum L.) and triticale (×Triticosecale Wittm.)’. Polish – Spanish research project (IPP PAS and Department of Genetics and Plant Breeding, EEAD-CSIC in Zaragoza); a role: researcher. 
2010- 2012 ‘Auxin as signalling molecule in doubled haploid production of rape (Brassica napus var. oleifera)’. Polish-Belgian research project (IPP PAS and VIB in Ghent University); a role: project coordinator.
2010 - 2012 ‘Molecular markers in auxin gradient analysis in androgenic embryos of rape (Brassica napus var. oleifera)’. Polish – Slovac research project (IPP PAS and Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences); a role: project coordinator.

INTERNATIONAL (FINISHED PROJECTS)

2010 - 2013 ‘Development methods to obtain differentiated morphologically and physiologically giant Miscanthus plants (Miscanthus x giganteus) by gyno-and  androgenesis’. Special project 1780 COST in the framework in Action COST FA0903; a role: researcher
2009 - 2011 ‘Localization of QTL for androgenic response on triticale (× Triticosecale Wittm.)’. Research project 548/N-COST/2009/0, COST Action FA0604: Triticeae genomics for the advancement of essential European crops (TritiGen); a role: researcher
2009 - 2011 ‘Physiological and biochemical indicators of winter rye resistance to Microdochium nivale’. Research project 592/N-COST/2009/0, COST Action FA0604: Triticeae genomics for the advancement of essential European crops (TritiGen); a role: researcher

COOPERATION WITH FOREIGN SCIENTIFIC INSTITUTIONS

2018 Research  fellowship at The Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia; 1 week
2016 Research  fellowship at The Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia; 1 week
2014 Research  fellowship at The Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia; 1 week
2012
  • Research fellowships at Plant Systems Biology Department (VIB), Ghent University, Ghent, Belgium; 4 weeks
  • Microspore embryogenesis in triticale (xTriticosecale Wittm.): assignment of genes to embryo formation" (individual research project, 2 weeks ) STMS of COST FA0903 “Harnessing plant reproduction for crop improvement” at EEAD-CSIC, Zaragoza, Spain, a role: the main researcher.
2011
  • Research fellowships at Plant Systems Biology Department (VIB), Ghent University, Ghent, Belgium; 3 weeks
  • Research  fellowship at The Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia; 1 week
  • Research fellowships at Plant Vegetal in (CSIC), Zaragoza in Spain; 2 weeks 
2010
  • Research fellowships at Plant Systems Biology Department (VIB), Ghent University, Ghent, Belgium; 1 WEEK
  • Research  fellowship at The Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia; 1 week
  • Research ‘NOBEL’ fellowships 'Transformation of microspores and androgenic embryos of rapeseed (Brassica napus)” in frame of “Investment into science – Investment into future. Joint project of three scientific centers from Krakow, Olsztyn and Warsaw” at The Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia; 1 week
  • Research ‘SAIA’ fellowships 'Techniques of recombinant DNA’ (individual research project) at The Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia; 4 weeks.
  • Research fellowships at Plant Systems Biology Department (VIB), Ghent University, Ghent, Belgium.
2009
  • Research fellowships at The Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia.
2008
  • Research fellowships at The Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia.
2003-2004
  • Research fellowships at Wageningen University and Research Centre in the frame of FP5 project CROPSTRESS and COST 851 Action STSM mission.

TEACHING EXPERIENCE

Universities
  Jagiellonian University in Krakow
2021 Lecture (titled "Auxins") for students of the 3rd degree at the Jagiellonian University in Krakow
2019 - 2020 
  • Lectures for undergraduate students in the Department of Biology, Jagiellonian University: Plant biology - basics (exercise) WBNZ-823, sem.: Z, hrs: 104 Plant physiology (exercise) WBNZ-971, sem.: L, hrs: 64; master seminars
2015 - 2019
  • Lecture (titled "Auxins") for students of the 3rd degree at the Jagiellonian University in Krakow
2018
  • Lecture in the Doctoral Studies of IB UJ, Krakow, as part of the course for PhD students: 'New developments, discoveries in botany
  Pedagogical University of Krakow 
2015 - 2017

Lecture (entitled "Fluorescence and confocal microscopy") for students of UP in Krakow.

Contracted teaching hours (lectures and tutorials). Courses in the framework of the Postgraduate Studies with Molecular Biology and Biotechnology Elements at the Faculty of Geography and Biology of Pedagogical University in Krakow. Postgraduate studies were carried out within Task 14 ‘Activation of Studies with Molecular Biology and Biotechnology Elements’ in the project ‘Developing the Didactic Potential in the Pedagogical University of Krakow’ co-founded by the European Social Funds in the academic years 2009/10 i 2010/11.

Others
  Polish Academy of Sciences in Krakow
2019 'Modern analytical techniques in research on haploidization of plants' - Lectures and exercises in English within the course 'Modern analytical techniques in research on haploidization of plants' for the participants of the Doctoral Studies in Natural Sciences PAS in Krakow; course coordinator
2019 Lectures and exercises in English within the course "Modern analytical techniques in plant haploidization studies" for participants of the PhD Studies in Natural Sciences PAS in Krakow; course coordinato
2016 - 2019 Lectures for participants of the Doctoral Studies in Natural Sciences PAS in Krakow
2017

Workshops for students of the Postgraduate Course "Modern methods in plant improvement", UR in Krakow, Faculty of Agriculture and Economics.

Lectures in the framework of the International Doctoral Studies of the Natural Sciences of the PAS in Krakow in 2014.

SCIENTIFIC SUPERVISION 

SUPERVISION OF Ph.D. STUDENTS

2020-now

Doctoral supervisor of the doctoral dissertation; Assistant supervisor Kenji Yamada, PhD (Malopolska Center of Biotechnology); Program: Doctoral School of Exact and Natural Sciences, Biomedical Sciences at the Jagiellonian University, Krakow; Thesis title: 'Plant chemical defense based on mustard oil bomb'. 

2021

Doctoral supervisor of the doctoral dissertation,  SDNP PAN in Cracow, thesis title: 'The stress-related molecular factors important for non-zygotic embryogenesis induction'. Date of defense: March 24, 2021>> https://> see below. The Doctoral Studies of the Natural Sciences of the PAS in Krakow.

  https://ibpan_krakow.bip.gov.pl/postepowania-doktorskie/mgr-inz-kamil-zielinski.html                                             

2017

Co-Promotor of the doctoral dissertation at Agriculture University in Krakow. Title of dissertation: ‘Utilization of androgenesis, gynogenesis and polyploidisation in the restoration of fertility in giant Miscanthus (Miscanthus x giganteus Greef et Deu.) plants’. Promotor: Prof. dr hab. Agnieszka Płażek, Agriculture University in Krakow.

 

SUPERVISION OF M.Sc STUDENTS

- Place: The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences, Department of Cell Biology:

2013 MSc Supervisor of two students: M.Sc Anna Malinowska and M.Sc Monika Okarmus in the Pedagogical University in Krakow, Faculty of Geography and  Biology, Institute of Biology.
2010-2012 Supervision of the execution experiments for two M.Sc students in the Institute of Biology at the Pedagogical University in Krakow
2007-2008 Supervision of the execution experiments for two M.Sc students in the Institute of Biology at the Pedagogical University in Krakow.

 

AWARDS AND PRIZES

2007 Doctoral dissertation with distinction at the Jagiellonian University in Krakow.
2011-2013 Award (2013) and distinctions (2011-2013) from the Director of the Institute of Plant Physiology Polish Academy of Sciences for the highest number of publications in journals from the Philadelphia list in the years  in the group of younger scientists.

 

THE PRACTICAL APPLICATIONS

  

2018-2019 Implementations of rye (Secale cereale L.) DH lines to Polish breeding programs.

Since 2015,  IPP PAS cooperates with Polish breeding and seed Companies (DANKO Hodowla Roślin, Sp. Z oo; DANKO Hodowla Roślin Sp. Z oo, 64 000 Kościan, Choryń 27; Poznańska Hodowla Roślin Sp. Z oo ul. Kasztanowa 5, 63 -004 Tulce) by introducing barley, oat and rye DH lines into the  traditional breeding programs; e.g. the seeds of rye DH lines were provided in order to restore the fertility of the hybrid rye.