Dr hab Ewa Dubas
Department of Cell Biology
(+48) 12 4253301 (wew./ext. 39)
e.dubas@ifr-pan.edu.pl
Publications
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- Żur I.
- Adamus A.
- Cegielska-Taras T.
- Cichorz S.
- Dubas E.
- Gajecka M.
- Juzoń-Sikora K.
- Kiełkowska A.
- Malicka M.
- Oleszczuk S.
- Skrzypek E.
- Szała L.
- Szarejko I.
- Zimny J.
- Doubled haploids: Contributions of poland’s academies in recognizing the mechanism of gametophyte cell reprogramming and their utilization in breeding of agricultural and vegetable species.
- 2022.
- Acta Societatis Botanicorum Poloniae, 91: 9128.
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- Dubas E.
- Castillo A.M.
- Żur I.
- Krzewska M.
- Vallés M.P.
- Microtubule organization changes severely after mannitol and n-butanol treatments inducing microspore embryogenesis in bread wheat.
- 2021.
- BMC Plant Biology, 21: 586 .
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- Dubas E.
- Żur I.
- Moravčiková J.
- Fodor J.
- Krzewska M.
- Surówka E.
- Nowicka A.
- Gerši Z.
- Proteins, small peptides and other signaling molecules identified as inconspicuous but possibly important players in microspores reprogramming toward embryogenesis.
- 2021.
- Frontiers in Sustainable Food Systems, 5: 745865.
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- Krzewska M.
- Dubas E.
- Gołębiowska G.
- Nowicka A.
- Janas A.
- Zieliński K.
- Surówka E.
- Kopeć P.
- Mielczarek P.
- Żur I.
- Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× Triticosecale Wittm.) after 5-azacytidine treatment.
- 2021.
- Scientific Reports, 11: 22215.
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- Dubas E.
- Zieliński K.
- Krzewska M.
- Żur I.
- Nowicka A.
- Kopeć P.
- Marcińska I.
- Czyczyło-Mysza I.
- Skrzypek E.
- Identyfikacja czynników determinujących efektywność otrzymywania podwojonych haploidów żyta (Secale cereale L.) metodami androgenezy i krzyżowań oddalonych.
- 2021.
- Biuletyn IHAR, 295: 185–188.
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- Żur I.
- Dubas E.
- Krzewska M.
- Nowicka A.
- Kopeć P.
- Hura T.
- Ostrowska A.
- Malaga S.
- Janeczko A.
- Gołębiowska-Pikania G.
- Rapacz M.
- Wójcik-Jagła M.
- Identyfikacja czynników determinujących odporność jęczmienia ozimego (Hordeum vulgare L.) na suszę i mróz.
- 2021.
- Biuletyn IHAR, 295: 139–144.
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- Żur I.
- Kopeć P.
- Surówka E.
- Dubas E.
- Krzewska M.
- Nowicka A.
- Janowiak F.
- Juzoń K.
- Janas A.
- Barna B.
- Fodor J.
- Impact of ascorbate-glutathione cycle components on the Effectiveness of embryogenesis induction in isolated microspore cultures of barley and triticale.
- 2021.
- Antioxidants, 10: 1254.
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- Żur I.
- Gajecka M.
- Dubas E.
- Krzewska M.
- Szarejko I.
- Albino plant formation in androgenic cultures: An old problem and new facts.
- 2021.
- Segui-Simarro J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2288. Humana, New York, NY.
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- Żur I.
- Dubas E.
- Krzewska M.
- Kopeć P.
- Nowicka A.
- Surówka E.
- Gawrońska K.
- Gołębiowska G.
- Juzoń K.
- Malaga S.
- Triticale and barley microspore embryogenesis induction requires both reactive oxygen species generation and efficient system of antioxidative defence.
- 2021.
- Plant Cell Tiss Organ Cult, 145: 347–366.
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- Zieliński K.
- Dubas E.
- Gerši Z.
- Krzewska M.
- Janas A.
- Nowicka A.
- Matušíková I.
- Żur I.
- Sakuda S.
- Moravčíková J.
- β-1,3-glucanases and chitinases participate in the stress-related defence mechanisms that are possibly connected with modulation of arabinogalactan proteins (AGP) required for the androgenesis initiation in rye (Secale cereale L.).
- 2021.
- Plant Science, 302: 110700.
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- Sychta K.
- Dubas E.
- Yamada K.
- Słomka A.
- Krzewska M.
- Kuta E.
- Papain-like cysteine proteases are involved in programmed cell death in plants under heavy metal stress.
- 2020.
- Environmental and Experimental Botany, 174: 104041.
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- Wójcik-Jagła M.
- Rapacz M.
- Dubas E.
- Krzewska M.
- Kopeć P.
- Nowicka A.
- Ostrowska A.
- Malaga S.
- Żur I.
- Candidate genes for freezing and drought tolerance selected on the basis of proteome analysis in doubled haploid lines of barley.
- 2020.
- International Journal of Molecular Sciences, 21: 2062.
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- Zieliński K.
- Krzewska M.
- Żur I.
- Juzoń K.
- Kopeć P.
- Nowicka A.
- Moravčiková J.
- Skrzypek E.
- Dubas E.
- The effect of glutathione and mannitol on androgenesis in anther and isolated microspore cultures of rye (Secale cereale L.).
- 2020.
- Plant Cell, Tissue and Organ Culture, 140: 577–592 .
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- Malaga S.
- Janeczko A.
- Janowiak F.
- Waligórski P.
- Oklestkova J.
- Dubas E.
- Krzewska M.
- Nowicka A.
- Surówka E.
- Rapacz M.
- Wójcik-Jagła M.
- Kopeć P.
- Hura T.
- Ostrowska A.
- Kaczanowska K.
- Żur I.
- Involvement of homocastasterone, salicylic and abscisic acids in the regulation of drought and freezing tolerance in doubled haploid lines of winter barley.
- 2020.
- Plant Growth Regulation, 90: 173–188.
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- Żur I.
- Dubas E.
- Malaga S.
- Janowiak F.
- Janeczko A.
- Rapacz M.
- Hura T.
- Waligórski P.
- Ostrowska A.
- Wójcik-Jagła M.
- Identyfikacja czynników determinujących odporność jęczmienia ozimego (Hordeum vulgare L.) na suszę i mróz.
- 2019.
- Biuletyn IHAR, 286: 113-116.
-
- Dubas E.
- Zieliński K.
- Krzewska M.
- Żur I.
- Nowicka A.
- Juzoń K.
- Identyfikacja czynników determinujących efektywność otrzymywania podwojonych haploidów żyta (Secale cereale L.) metodami androgenezy i krzyżowań oddalonych.
- 2019.
- Biuletyn IHAR, 286: 135-137.
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- Nowicka A.
- Juzoń K.
- Krzewska M.
- Dziurka M.
- Dubas E.
- Kopeć P.
- Zieliński K.
- Żur I.
- Chemically-induced DNA de-methylation alters the effectiveness of microspore embryogenesis in triticale.
- 2019.
- Plant Science, 287: 110189.
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- Żur I.
- Dubas E.
- Krzewska M.
- Zieliński K.
- Fodor J
- Janowiak F.
- Glutathione provides antioxidative defence and promotes microsporederived embryo development in isolated microspore cultures of triticale (×Triticosecale Wittm.).
- 2019.
- Plant Cell Reports, 38: 195–209.
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- Hura T.
- Katarzyna Hura
- Ostrowska A.
- Gadzinowska J.
- Grzesiak M.
- Dziurka K.
- Dubas E.
- Rieske iron-sulfur protein of cytochrome-b6f is involved in plant recovery after drought stress.
- 2018.
- Environmental and Experimental Botany, 156: 228-239.
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- Marcińska I.
- Czyczyło-Mysza I.
- Skrzypek E.
- Warchoł M.
- Zieliński K.
- Dubas E.
- Obtaining of winter rye (Secale cereale L.ssp. cereale) haploid embryos through hybridization with maize (Zea mays L.).
- 2018.
- Cereal Research Communications, 46: 521–532.
Projects
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MRiRW nr 4,
Identyfikacja czynników warunkujących indukcję embriogenezy mikrospor u pszenicy zwyczajnej (Triticum aestivum L.) -
Tango2/340285/NCBR/2017,
Protocol for effective doubled haploids production by androgenesis for polish breeding materials of wheat (Triticum aestivum L.) -
2015/18/M/NZ3/00348,
Oxidative stress and its role in the regulation of embryogenesis in isolated microspore cultures of triticale (x Triticale Wittm.) and barley (Hordeum vulgare L.) -
MRiRW, nr 84,
Identification of factors determining the efficiency of doubled haploids production in rye (Secale cereale L.) through androgenesis and distant crosses -
MRiRW, nr 26,
Identification of factors which determine drought and freezing tolerance in barley (Hordeum vulgare L.)
DIPLOMAS, DEGREES
2015- present | Associate Professor in Agriculture, Agriculture University, Kraków, Poland |
2007- | PhD in Biology, Jagiellonian University, Kraków, Poland |
2001- | M.Sc in Biology – Department of Plant Cytology and Embryology, Jagiellonian University, Krakow, Poland |
EMPLOYMENT IN ACADEMIC INSTITUTIONS
2020-present |
Associate Professor, Department of Cell Biology in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow |
2020-2020 |
Adiunct [Assistant Professor], Department of Plant Cytology and Embryology, Jagiellonian University, Krakow |
2015-2019 |
Associate Professor, Department of Cell Biology in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow |
2007-2015 |
Adiunct [Assistant Professor], Department of Cell Biology in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow |
2001-2007 |
PhD student, Department of Cell Biology in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow |
2001-2007 |
Interdisciplinary Ph.D. Study in the Faculty of Biology and Earth Sciences, Jagiellonian University |
MEMBERSHIP AND FUNCTIONS
2020-now | Member of the Scientific Council in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow |
2019-2020 | Member of the Scientific Council of the Faculty of Biology of the Jagiellonian University in Krakow |
2015-2019 | Member of the Scientific Council in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow |
2014-2017 | Polish deputy in Management Committee of COST FA1306 ‘The quest for tolerant varieties: 'Phenotyping at plant and cellular level’ |
2014 | External expert evaluator of the international research projects Fund for Scientific Research – FNRS; Slovak Research and Development Agency; Czech National Agency |
2011-2013 | Polish deputy in Management Committee of COST FA (Food and Agriculture) 0903 ‘Harnessing Plant Reproduction for Crop Improvement’ |
RESEARCH AREAS
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PRACTICAL SKILLS
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RESEARCH EXPERIENCE
Post doc projects. My Post Doc projects concentrated mainly on: (1) endogenous auxins as a triggers in double haploids (DHs) production in oilseed rape, (2) the role of polar auxin transport and auxin signalling in embryo development. Plant embryonic development involves differential distribution (gradients) of the plant signaling molecule auxin. These gradients are established and maintained by a directional, intercellular auxin transport called polar auxin transport. Polar auxin transport provides positional and directional information for many aspects of embryo development. B. napus microspore suspension is a suitable model system for such studies. By using combination of genetic, molecular, physiological and cell biology approaches I would like to address the key question: how plant hormones perform their respective roles in embryo development, with a special focus on hormonal interactions. |
PhD project. My PhD project concentrated on early embryo development in microspore suspension in vitro cultures of Brassica napus. High yields of microspore-derived embryos MDE in suspension were reproducibly obtained according to the method elaborated previously by research team of dr Jan Custers PRI Wageningen, The Netherlands. I adopted the method and further optimised in my laboratory. MDE develop and differentiate in a way resembling zygotic embryos in almost every studied aspect, therefore I used them to study early stages of plant embryogenesis. The main goal of these studies was to study the development of androgenic embryos from the earliest stages (a single cell) onwards including suspensor formation. Studying those structures during zygotic development is very difficult due to ovular tissues that surround embryos. I focused on establishment of embryo polarity and the role of cytoskeleton during MDE differentiation tracing also mitochondria and amyloplasts positions, vacuolisation pattern, cell-wall formation and differentiation of embryo organs. My studies required optimalization of many methods and development of protocols of immunodetection and tracing of various structures in whole-mount (not sectioned) material. |
PUBLICATIONS
Selected older papers (up to 2017) listed by year with links to the publishers' websites:
2015-2018
COORDINATION/PARTICIPATION IN RESEARCH PROJECTS
NATIONAL (RUNNING PROJECTS)
NATIONAL (FINISHED PROJECTS)
BILATERAL (FINISHED PROJECTS)
INTERNATIONAL (FINISHED PROJECTS)
COOPERATION WITH FOREIGN SCIENTIFIC INSTITUTIONS
TEACHING EXPERIENCE
SCIENTIFIC SUPERVISION
SUPERVISION OF Ph.D. STUDENTS
2020-now |
Doctoral supervisor of the doctoral dissertation; Assistant supervisor Kenji Yamada, PhD (Malopolska Center of Biotechnology); Program: Doctoral School of Exact and Natural Sciences, Biomedical Sciences at the Jagiellonian University, Krakow; Thesis title: 'Plant chemical defense based on mustard oil bomb'. |
2021 |
Doctoral supervisor of the doctoral dissertation, SDNP PAN in Cracow, thesis title: 'The stress-related molecular factors important for non-zygotic embryogenesis induction'. Date of defense: March 24, 2021>> https://> see below. The Doctoral Studies of the Natural Sciences of the PAS in Krakow. https://ibpan_krakow.bip.gov.pl/postepowania-doktorskie/mgr-inz-kamil-zielinski.html |
2017 |
Co-Promotor of the doctoral dissertation at Agriculture University in Krakow. Title of dissertation: ‘Utilization of androgenesis, gynogenesis and polyploidisation in the restoration of fertility in giant Miscanthus (Miscanthus x giganteus Greef et Deu.) plants’. Promotor: Prof. dr hab. Agnieszka Płażek, Agriculture University in Krakow. |
SUPERVISION OF M.Sc STUDENTS
- Place: The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences, Department of Cell Biology:
2013 | MSc Supervisor of two students: M.Sc Anna Malinowska and M.Sc Monika Okarmus in the Pedagogical University in Krakow, Faculty of Geography and Biology, Institute of Biology. |
2010-2012 | Supervision of the execution experiments for two M.Sc students in the Institute of Biology at the Pedagogical University in Krakow |
2007-2008 | Supervision of the execution experiments for two M.Sc students in the Institute of Biology at the Pedagogical University in Krakow. |
AWARDS AND PRIZES
2007 | Doctoral dissertation with distinction at the Jagiellonian University in Krakow. |
2011-2013 | Award (2013) and distinctions (2011-2013) from the Director of the Institute of Plant Physiology Polish Academy of Sciences for the highest number of publications in journals from the Philadelphia list in the years in the group of younger scientists. |
THE PRACTICAL APPLICATIONS
2018-2019 | Implementations of rye (Secale cereale L.) DH lines to Polish breeding programs. |
Since 2015, IPP PAS cooperates with Polish breeding and seed Companies (DANKO Hodowla Roślin, Sp. Z oo; DANKO Hodowla Roślin Sp. Z oo, 64 000 Kościan, Choryń 27; Poznańska Hodowla Roślin Sp. Z oo ul. Kasztanowa 5, 63 -004 Tulce) by introducing barley, oat and rye DH lines into the traditional breeding programs; e.g. the seeds of rye DH lines were provided in order to restore the fertility of the hybrid rye.