Dr hab Piotr Waligórski

Role and mechanisms of mycorrhiza in plants and its contribution to alleviation of drought stress Proteomics of galls

Publications

    • Jakovljević D.
    • Kruszka D.
    • Waligórski P.
    • Warchoł M.
    • Skrzypek E.
    • Untargeted metabolomic in basil cell cultures – a case study of Ocimum basilicum L. var. minimum Alef..
    • 2024.
    • Physiologia Plantarum, 176: e14203.
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    • Wojtania A.
    • Markiewicz M.
    • Waligórski P.
    • Growth cessation and dormancy induction in micropropagated plantlets of Rheum rhaponticum ‘Raspberry’ influenced by photoperiod and temperature.
    • 2023.
    • International Journal of Molecular Sciences, 24: 607.
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    • Konieczna W.
    • Warchoł M.
    • Mierek-Adamska A.
    • Skrzypek E.
    • Waligórski P.
    • Piernik A.
    • Dąbrowska G.B.
    • Changes in physio-biochemical parameters and expression of metallothioneins in Avena sativa L. in response to drought.
    • 2023.
    • Scientific Reports, 13: 2486.
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    • Morańska E.
    • Simlat M.
    • Warchoł M.
    • Skrzypek E.
    • Waligórski P.
    • Laurain-Mattar D.
    • Spina R.
    • Ptak A.
    • Phenolic acids and Amaryllidaceae alkaloids profiles in Leucojum aestivum L. in vitro plants grown under different light conditions.
    • 2023.
    • Molecules, 28: 1525.
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    • Wojtkiewicz A.M.
    • Glanowski M.
    • Waligórski P.
    • Janeczko T.
    • Szaleiec M.
    • 1,2-hydrogenation and transhydrogenation catalyzed by 3-ketosteroid δ1-dehydrogenase from Sterolibacterium denitrificans - Kinetics, isotope labelling and QM:MM modelling studies.
    • 2022.
    • International Journal of Molecular Sciences, 23: 14660 .
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    • Wojtania A.
    • Markiewicz M.
    • Waligórski P.
    • Regulation of the bud dormancy development and release in micropropagated rhubarb 'Malinowy'.
    • 2022.
    • International Journal of Molecular Sciences, 23: 1480.
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more publications

Projects

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EDUCATION AND CAREER

2018 - present - assistant at The Franciszek Gorski Institute of Plant Physiology, Polish Academy of Science, Krakow, Poland
2008 – 2018 - post-doc (adiunkt) at The Franciszek Gorski Institute of Plant Physiology, Polish Academy of Science, Krakow, Poland
2014 - present - head of The Joint Laboratory of Biotechnology and Enzyme Catalysis
2005 – 2014 - staff member at The Joint Laboratory of Biotechnology and Enzyme Catalysis
2010 - 2011 - Postdoctoral Researcher at Stanley S. Scott Cancer Center, Louisiana State University, New Orleans, USA
2004 – 2008 - assistant at The Franciszek Gorski Institute of Plant Physiology, Polish Academy of Science, Krakow, Poland
1998 – 2003 - Ph.D. Studies at the Faculty of Agriculture and Economics, Agricultural University, Krakow, Poland
1993 – 1998 - M.Sc. Studies at the Faculty of Biology, Jagiellonian University, Krakow, Poland, speciality: molecular biology


RESEARCH INTERESTS

Hormonal regulation of plant flowering.
Proteomics of galls.
Physiological role of silicon compounds.
Self-incompatibility of cabbage.
Arbuscular mycorrhiza and its link with hormonal regulation of plant physiology.
The application of modern techniques in plant physiology studies: high performance liquid chromatography, mass spectroscopy, capillary electrophoresis.

PRACTICAL SKILLS

Determination of the plant compounds (MS HPLC):
- auxines (IAA, IBA)
- cytokinines (kinetin, zeatin, their rybosides and others)
- abscisic acid (ABA)
- polyamines (putrescine, spermine, spermidine and others)
- sugars (glucose, fructose, sucrose, maltose and others)
- phenolic acids (salicylic acid, caffeic, cynnamic and others)
- carotenoids (violaxanthin, zeaxanthin, lutein and others)
- flavonoids
- tocochromanoles (tocopheroles and tocotrienoles)

Capillary Electrophoresis:
- proteins
- organic and inorganic anions

Proteomics
- 2D gel electrophoresis
- MALDI TOF protein identification
- ShotGun analyses

ELISA assay

Image analysis:
- good experience in LUCIA system

PARTICIPATION IN GRANTS
N N310 452638 - Możliwość zwiększenia efektywności androgenezy u pszenżyta (x Triticosecale Wittm.) poprzez kontrolę równowagi hormonalnej i natężenia stresu oksydacyjnego w pylnikach. Project leader: dr hab. Iwona Żur 2010 - 2013
621/N-COST/09/2010/0 - Aktywność poliamin i związków fenolowych w reakcji odporności na suszę glebową roślin motylkowych uprawianych na białko. Project leader: dr inż. Edyta Skrzypek, 2010 - 2012
2 P04G 109 28 – The influence of mycorrhizal fungi on biomass production and level of active compounds in Arnica montana L. Project leader: dr Anna Jurkiewicz, 23.03.2005-22.03.2008
CROPSTRESS QLAM-2001-00424 – Centre of Research on the Biology of Plants Subjected to Environmental Stress in Sustainable Agricultural Production. Project leader: dr hab. Maria Wędzony, 01.04.2003-30.03.2006
R12 045 02 – Development of methods of vegetable treating for increasing its global resistance, content of natural antioxidants and stabilization of crop quality during storage. Project leader: prof. dr hab. Zbigniew Miszalski
PL 023168 SAFIR – Safe and High Quality Food Production using Low Quality Waters and Improved Irrigation Systems and Management. 2006-2008
5 P06A 032 18 – The influence of coincidence between photosynthetic active radiation and temperature to gaining frost resistance in rape (Brassica napus var. oleifera) by growth rate changes during prehardening and dehardening. Project leader: dr eng. Marcin Rapacz, 01.01.2000-31.12.2002