Dr Monika Krzewska

Department of Cell Biology
(+48) 12 4253301 (wew./ext. 39)
Physiological and molecular aspects of microspore embryogenesis • doubled haploid production (DH) • quantitative trait loci (QTL) analysis


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2003-2008  study at the Faculty of Geography and Biology, Pedagogical Academy (now University of the National Education Commision) in Kraków (UNEC)
2008 master degree in biological sciences with teaching of nature and health education at UNEC
2009 - 2013 PhD student in International Doctoral Studies in Natural Sciences at the Polish Academy of Sciences in Kraków
2013 doctor’s degree in agricultural sciences, speciality: agronomy, plant physiology at The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences in Kraków (IPP PAS)


2009-2010 technician at UNEC
2008-2013 technician at IPP PAS
since 2013 adiunkt at IPP PAS


  • In vitro culture techniques in cereals - methods for doubled haploid (DH) production.
  • Quantitative Trait Loci (QTL) analysis for androgenic responsiveness.
  • The proteomic analysis of androgenic responsiveness


  • The methods of in vitro cultures
  • QTL Analysis - software WinQTLCartographer v 2.5
  • Two dimensional elecrtophoresis (2DE)
  • The protein profiles comparision - PDQuest v. 8.0


Krzewska M., Dubas E., Gołębiowska G., Nowicka A., Janas A., Zieliński K., Surówka E., Kopeć P., Mielczarek P., Żur I. (2021) Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× Triticosecale Wittm.) after 5-azacytidine treatment. Scientific Reports, 11: 22215. doi: 10.1038/s41598-021-01671-y 

Dubas E., Castillo A.M., Żur I., Krzewska M., Vallés M.P. (2021) Microtubule organization changes severely after mannitol and n-butanol treatments inducing microspore embryogenesis in bread wheat. BMC Plant Biol, 21: 586. doi: 10.1186/s12870-021-03345-3 

Dubas E., Żur I., Moravčíková J., Fodor J., Krzewska M., Surówka E., Nowicka A., Gerši Z. (2021) Proteins, Small Peptides and Other Signaling Molecules Identified as Inconspicuous but Possibly Important Players in Microspores Reprogramming Toward Embryogenesis. Front. Sustain. Food Syst., 5: 745865. doi: 10.3389/fsufs.2021.745865 

Surówka E., Latowski D., Dziurka M., Rys M., Maksymowicz A., Żur I., Olchawa-Pajor M., Desel C., Krzewska M., Miszalski Z. (2021) ROS-Scavengers, Osmoprotectants and Violaxanthin De-Epoxidation in Salt-Stressed Arabidopsis thaliana with Different Tocopherol Composition. Int. J. Mol. Sci., 22: 11370. doi: 10.3390/ijms222111370 

Żur I., Kopeć P., Surówka E., Dubas E., Krzewska M., Nowicka A., Janowiak F., Juzoń K., Janas A., Barna B., Fodor J. (2021) Impact of ascorbate-glutathione cycle components on the Effectiveness of embryogenesis induction in isolated microspore cultures of barley and triticale. Antioxidants, 10: 1254. doi: 10.3390/antiox10081254 

Żur I., Dubas E., Krzewska M., Kopeć P., Nowicka A., Surówka E., Gawrońska K., Gołębiowska G., Juzoń K., Malaga S. (2021) Triticale and barley microspore embryogenesis induction requires both reactive oxygen species generation and efficient system of antioxidative defence. Plant Cell Tiss Organ Cult, 145: 347–366. doi: 10.1007/s11240-021-02012-7 

Zieliński K., Dubas E., Gerši Z., Krzewska M., Janas A., Nowicka A., Matušíková I., Żur I., Sakuda S., Moravčíková J. (2021) β-1,3-glucanases and chitinases participate in the stress-related defence mechanisms that are possibly connected with modulation of arabinogalactan proteins (AGP) required for the androgenesis initiation in rye (Secale cereale L.). Plant Science, 302: 110700. doi: 10.1016/j.plantsci.2020.110700 

Sychta K., Dubas E., Yamada K., Słomka A., Krzewska M. Kuta E. (2020) Papain-like cysteine proteases are involved in programmed cell death in plants under heavy metal stress.Environmental and Experimental Botany, 174: 104041. doi:10.1016/j.envexpbot.2020.104041 

Wójcik-Jagła M., Rapacz M., Dubas E., Krzewska M., Kopeć P., Nowicka A., Ostrowska A., Malaga S., Żur I. (2020) Candidate genes for freezing and drought tolerance selected on the basis of proteome analysis in doubled haploid lines of barley. International Journal of Molecular Sciences, 21: 2062. doi: 10.3390/ijms21062062 

Zieliński K., Krzewska M., Żur I., Juzoń K., Kopeć P., Nowicka A., Moravčiková J., Skrzypek E. Dubas E. (2020) The effect of glutathione and mannitol on androgenesis in anther and isolated microspore cultures of rye (Secale cereale L.). Plant Cell, Tissue and Organ Culture, 140: 577–592. doi:10.1007/s11240-019-01754-9 

Malaga, S., Janeczko, A., Janowiak, F., Waligórski P., Oklestkova J., Dubas E., Krzewska M., Nowicka A., Surówka E., Rapacz M., Wójcik-Jagła M., Kopeć P., Hura T., Ostrowska A., Kaczanowska K., Żur I.(2019) Involvement of homocastasterone, salicylic and abscisic acids in the regulation of drought and freezing tolerance in doubled haploid lines of winter barley. Plant Growth Regul, 90: 173–188. doi: 10.1007/s10725-019-00544-9 

Nowicka A., Juzoń K., Krzewska M., Dziurka M., Dubas E., Kopeć P., Zieliński K., Żur I. (2019) Chemically-induced DNA de-methylation alters the effectiveness of microspore embryogenesis in triticale. Plant science : an international journal of experimental plant biology 287:110189. doi: 10.1016/j.plantsci.2019.110189 

Żur I., Dubas E., Krzewska M., Zieliński K., Fodor J., Janowiak F. (2019). Glutathione provides antioxidative defence and promotes microspore-derived embryo development in isolated microspore cultures of triticale (× Triticosecale Wittm.). Plant Cell Reports. doi: 10.1007/s00299-018-2362-x

Gołębiowska-Pikania G., Kopeć P., Surówka E., Janowiak F., Krzewska M., Dubas E., Nowicka A., Kasprzyk J., Ostrowska A., Malaga S., Hura T., Żur I. (2017). Changes in protein abundance and activity induced by drought during generative development of winter barley (Hordeum vulgare L.) Journal of Proteomics 169:73-86, doi: 10.1016/j.jprot.2017.07.016

Gołębiowska-Pikania G., Kopeć P., Surówka E., Krzewska M., Dubas E., Nowicka A., Rapacz M., Wójcik-Jagła M., Malaga S., Żur I. (2017). Changes in protein abundance and activity involved in freezing tolerance acquisition in winter barley (Hordeum vulgare L.), Journal of Proteomics 169:58-72, doi: 10.1016/j.jprot.2017.08.019

Krzewska M., Gołębiowska-Pikania G., Dubas E., Gawin M., Żur I. (2017). Identification of proteins related to microspore embryogenesis responsiveness in winter triticale (×Triticosecale Wittm.). Euphytica 213:192. doi: 10.1007/s10681-017-1978-1

Krzewska M., Czyczyło-Mysza I., Dubas E., Gołębiowska-Pikania G., Żur I. (2015). Identification of QTLs associated with albino plant formation and some new facts concerning green versus albino ratio determinants in triticale (×Triticosecale Wittm.) anther culture. Euphytica 206: 263-278. doi: 10.1007/s10681-015-1509-x

Żur I., Dubas E., Krzewska M., Janowiak F. (2015). Current insights into hormonal regulation of microspore embryogenesis. Frontiers in Plant Science 6 doi:10.3389/fpls.2015.00424

Żur I., Dubas E., Krzewska M., Waligórski P.,  Dziurka M.,  Janowiak F. (2015). Hormonal requirements for effective induction of microspore embryogenesis in triticale (× Triticosecale Wittm.) anther cultures Plant Cell Reports, 34:47-62, doi:10.1007/s00299-014-1686-4

Dubas E., Moravcikova J., Libantova J., Matusikova I., Benkova E., Zur I., Krzewska M. (2014). The influence of heat stress on auxin distribution in transgenic B. napus microspores and microspore-derived embryos. Protoplasma, 251(5):1077-1087. doi: 10.1007/s00709-014-0616-1 PUBLIKACJE Z

Żur I., Dubas E., Krzewska M., Janowiak F., Hura K., Pociecha E., Bączek-Kwinta R., Płażek A. (2014). Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis. Plant Cell, Tissue and Organ Culture (PCTOC), 119(1):79-94. doi: 10.1007/s11240-014-0515-3

Żur I., Dubas E., Krzewska M., Sánchez-Díaz R. A., Castillo A. M., Vallés M. P. (2014). Changes in gene expression patterns associated with microspore embryogenesis in hexaploid triticale (×Triticosecale Wittm.). Plant Cell, Tissue and Organ Culture (PCTOC), 116(2):261-267. doi: 10.1007/s11240-013-0399-7

Dubas E., Janowiak F., Krzewska M., Hura T., Zur I. (2013). Endogenous ABA concentration and cytoplasmic membrane fluidity in microspores of oilseed rape (Brassica napus L.) genotypes differing in responsiveness to androgenesis induction. Plant Cell Rep, 32(9):1465-1475. doi: 10.1007/s00299-013-1458-6

Krzewska M., Czyczylo-Mysza I., Dubas E., Golebiowska-Pikania G., Golemiec E., Stojalowski S., Chrupek M., Zur I. (2012). Quantitative trait loci associated with androgenic responsiveness in triticale (xTriticosecale Wittm.) anther culture. Plant Cell Rep, 31(11):2099-2108. doi: 10.1007/s00299-012-1320-2

Żur I., Krzewska M., Dubas E., Gołębiowska-Pikania G., Janowiak F., Stojałowski S. (2012). Molecular mapping of loci associated with abscisic acid accumulation in triticale (×Triticosecale Wittm.) anthers in response to low temperature stress inducing androgenic development. Plant Growth Regulation, 68(3):483-492. doi: 10.1007/s10725-012-9738-7

More articles:



26-30.09.2010. Slovakia (Nitra) at the Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences of the Polish - Slovak research project: "The search for protein resistance to snow mold in winter triticale"

18-29.09.2011. Spain (Zaragozza) at Estación Experimental de Aula Dei Consejo Superior de investigaciones Científicas of the bilateral project: „N-butanol as a trigger inducing microspore embryogenesis in in vitro cultures of wheat (Triticum aestivum L.) and triticale (×Triticosecale Wittm.)

06-13.11.2011. Slovakia (Nitra) at the Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences of the bilateral project: "The search for protein resistance to snow mold in winter triticale"

26.11-7.12.2012. Slovakia (Nitra) at the Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences of the bilateral project: "The search for protein resistance to snow mold in winter triticale"