Is 4-chloro-indolyl-3-acetic acid the death hormone in generative oat organs?


Summary: The term "death hormone" appeared in the natural sciences at the end of the eighties of the last century (Engvild 1989) and mainly concerns halogenated auxins. So far, the mechanism of action of these phytohormones has not been fully elucidated. It is suggested that synthesized in generative organs and seeds may initiate the aging process of vegetative organs of monocarp plants. The representative of this group of hormones, 4-chloroindolyl-3-acetic acid (4Cl-IAA), was detected mainly in legume seeds (Reinecke 1999). Dziurka and others (2016) showed the presence of 4Cl-IAA in oat ovary three weeks after cross-breeding away from the corn, in the absence of a haploid embryo. It can be assumed that in the embryos in which the haploid embryo did not develop, the aging and dying process was initiated. The aim of the proposed studies will be to confirm the presence of 4Cl-IAA in generative oat organs, ie anthers and pistils, and to verify the hypothesis that 4Cl-IAA can induce the aging process of the above-mentioned organs. For this purpose, the analysis of 4Cl-IAA content will be carried out in immature and mature anthers of oats, as well as in non-dusted and dusted pollen bars (3, 7, 14 and 21 days after pollination). 4Cl-IAA will be determined by HPLCMS / MS (Dziurka et al 2016).